This Combined Synopsis/Solicitation opportunity from Department Of Health And Human Services was posted on July 9, 2026. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

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Acquisition of an Applied Biosystems QuantStudio 3 Real-Time PCR System

Closed

75N98026Q00714Federal

Submission Closed07/20/2026, 09:00:00 AM EDT

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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NAICS: 334516

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1 day ago

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in 5 days

AI Contract Overview

The National Institutes of Health, through the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), is seeking to acquire a single Applied Biosystems QuantStudio 3 Real-Time PCR System to replace aging StepOnePlus instruments and ensure uninterrupted research operations. This system is essential for performing nucleic acid quantification and analysis across a broad range of applications including gene expression, pathogen detection, and validation of experimental results. The instrument must match the StepOnePlus in workflow compatibility while offering enhanced capabilities such as touchscreen operation, standalone functionality, and support for both Mac and PC platforms. It must feature a 96-well 0.1 mL block with VeriFlex technology enabling up to a 10°C temperature gradient across separated zones, support reaction volumes of 10–30 μL, and execute fast PCR protocols under 30 minutes. The system must be factory-calibrated for key dyes, include a heated lid, and operate on standard 120–240 V power without modification. It must come with an onboard computer with 10 GB storage, support for USB, network, and Connect platform data transfers, and allow local user accounts with PIN access and guest mode functionality. The instrument must integrate seamlessly into the broader QuantStudio ecosystem, including cloud connectivity via the Connect platform for remote monitoring, data uploads, and batch analysis of up to 500 experiments. Software must support absolute and relative quantification, SNP genotyping, high-resolution melt analysis, and allow protocol conversion from StepOnePlus systems. It must include preoptimized templates, statistical tools like box-whisker and heat maps, and the ability to import standard curves from one plate to another. Data can be exported to Excel and saved as image files, with command-line functions for batch processing. The system must support more than 61,000 pre-designed TaqMan assays for human, mouse, and rat gene expression, and include an API for custom programming. The contractor must deliver and install the system within 30 days of award and accept trade-in of the existing StepOnePlus unit with serial number 272008444. The device must meet ISO 13485 and ISO 9001 manufacturing standards and hold regulatory certifications including UL, CE, and WEEE. All features are designed to ensure data consistency, operational efficiency, and long-term technical support.

General Info

NIAMS seeks QuantStudio 3 Real-Time PCR system to replace aging StepOnePlus, ensuring seamless workflow, fast PCR, cloud connectivity, and full compatibility.

Agency

Department Of Health And Human Services → National Institutes Of Health Olao

NAICS

334516 - Analytical Laboratory Instrument Manufacturing

Place of Performance

Bethesda, MD, 20892, USA

Set-Aside

NONE

Documents

sow

High

AI Contract Breakdown

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Timeline

PhaseClosed

PostedJul 9

Combined Synopsis

Response DeadlineJul 20

Deadline has passed

Submission Closed

07/20/2026, 09:00:00 AM EDT

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Organization & Contact Information

AgencyDepartment Of Health And Human Services → National Institutes Of Health Olao

Contacts1 person available

OfficeBETHESDA, MD, 20892, USA

Organization / AgencyDepartment Of Health And Human Services → National Institutes Of Health Olao

Office AddressBETHESDA, MD, 20892, USA

Contacts

John Burton

Full Description

Requirements:

To ensure continuity of research, NIAMS proposes the acquisition of an Applied Biosystems QuantStudio 3 Real-Time PCR System. qPCR is a core technology used for nucleic acid amplification, quantification, and analysis and supports a wide range of research applications, including gene expression studies, pathogen detection, transcript quantification, and validation of experimental results. The QuantStudio 3 is the closest replacement to the current StepOnePlus systems, allowing researchers to maintain established workflows and consistency in experimental design and data interpretation. The QuantStudio 3 offers several operational advantages, including user-friendly software, compatibility with both Mac and PC platforms, touchscreen operation, and the ability to function as a standalone instrument or in conjunction with a computer. Its thermal block design is similar to the existing systems, minimizing the learning curve for users and supporting a broad range of genomic applications. In addition, the manufacturer offers trade-in credit for the current instruments, reducing the overall acquisition cost while providing access to a fully supported platform with available parts and technical support. Acquiring the QuantStudio 3 at this time will help prevent interruptions to research activities, maintain reliable and consistent data generation, and ensure that NIAMS laboratories have access to a supported and dependable qPCR platform.

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS The contractor shall provide one Applied Biosystems QuantStudio 3 Real-Time PCR System or an equal system that meets all the following minimum requirements. 4.1.1. System Features/Benefits The system shall: • Utilize a platform providing 1 TB or storage space for data sharing at user discretion • be used with online scientific apps • come with desktop-based software, allowing single-plate run setup and analysis.

4.1.2. Technical specifications – Instrument The instrument shall: • have a 96 well 0.1 ml plate block format and the option to run fast protocols <30 minutes • not require a field service engineer for set up by end user. Set up should be easy for end user – directly out of the box. • not require a computer for installation • use an RNase P verification plate to verify performance • not require dye calibration upon installation • have dimensions fo 27 x 50 x 40 cm and be designed to maximize horizonal and vertical bench space • be capable of analyzing 96 samples simultaneously in various formats incluing both 96-well plates, 8-tube strips and individual tubes • the 96-well block should be made of at least 3 separated Peltier elements – VeriFlex Blocks. The maximum temperature difference programmable across the block should be 10 degrees C. 5 degrees C should be the maximum difference in temperature allowed between adjacent blocks. • Support reaction volumes of 10-30 uL on 0.1mL the 96-well block • Be factory calibrated with FAM, SYBR Green 1, VIC, NED, ABY, JUN, TAMRA, and ROX dyes • Have melt or dissociation curves that take place in steps with deltaT varying between 0.015 degrees C and 3.66 degrees C • Have a maximum block ramp rate of 9.0 degrees C/sec for the 0.1 mL block • Have a block with a temperature range of 4-99.9 degrees celcius • Have a heated cover with temperature range of 50-110 degreesC 2

• Have a long-lasting bright white LED source with median lifetime of at least 60,000 hours • Have a CMOS imager for data acquisition and collects data for each filter combination in less than 2 seconds • be used with 120-240 V outlets without modification or special plug requirements • Include a heated lid assembly to heat tops of sample plates and minimize reaction evaporation.

4.1.3 Technical specifications – Instrument onboard computer The system shall: • Possess the capability to run in stand-alone mode, without an attached computer • Include a touchscreen interface and store protocols for quick starts with no computer needed • Have a touchscreen interface that enables viewing amplification plots and filtering them by sample, target and task • Have a touchscreen interface that facilitates uploading of protocols or downloading of run files via a USB, local network and the Connect platform • Have a touchscreen interface that allows uploading of protocols and run files directly to the Connect platform • Contain onboard storage with memory capacity of 10 GB for storing 1,000-5,000 standard absolute quantification run files, and be designed for run protection in case of power outages • Be able to be customized through the touchscreen for time zones and service/calibration notification settings • Have a touchscreen interface that supports the creation of local user accounts with PIN-protected access that can be linked to the Connect platform to online accounts on thermofisher.com • Have a touchscreen interface that allows creation and running of protocols as guests • Have a touchscreen interface that allows for editing of protocols at all stages of the reaction cycle as well as block settings, temperature settings duration, ramp rate, etc. • Have a touchscreen interface that allows a template to be loaded from a USB, local network or the Connect platform • Have a touchscreen interface that allows user to enter reagent information • Have a touchscreen interface that allows a run to be paused and during that pause for the block temperature to be set as well as for the drawer to be opened and closed • Have a touchscreen interface that allows editing of sample and target assignments • Have a touchscreen interface that allows the repeat of a previous run 3 • Have a touchscreen interface that allows users to perform dye calibrations and view results on the touchscreen • Have a touchscreen interface that allows RNase P verification runs and automatically provides pass/fail results • Have a touchscreen interface that allows a user to run a self-verification test • Have a touchscreen interface that allows the user to view the cure QC assessment after calibration and RNase P plate runs are finished • Have a touchscreen interface that allows progress monitoring.

4.1.4 Performance Requirements The instrument shall: • Complete 40-cycle real-time PCR runs using fluorogenic 5’ nuclease assay and Fast chemistry in a standard 96-well plate in less than 30 minutes as well as being able to run in standard ramping mode with standard chemistry • Include an OptiFlex system that combines four coupled excitation, and emission filter sets to allow multiplexing up to four unique wavelengths • Support at least two reaction chemistries-TaqMan® Probes and SYBR Green dye • Have real-time quantitative PCR installation specifications to be able to distinguish between 5,000 and 10,000 template copies at a 99.7% confidence level.

4.1.5 Software Requirements The software shall: • Allow flexible customization of run protocols • Allow the creation of secure templates that are locked with a password to limit modification of run setup • Provide run monitoring of connected instruments • Include preoptimized run protocols for applications like standard curve, relative standard curve, relative quantification of gene expression, and SNP genotyping with TaqMan® or SYBR Green chemistries • Include a copy of free software to analyze data for absolute and relative quantification, SNP detection and other assays. • Collect information on both TaqMan and SYBR assays in the same run • Have a genotyping optimization tool that uses real-time data • Provide raw fluorescence data and multicomponent data for troubleshooting • Allow conversion of protocols for the Applied Biosystems StepOnePlus and 7500 to the QuantStudio 3 system protocols • Allow batch analysis of up to 500 experiments simultaneously • Can control the instrument and analyze data from a remote computer on the same network 4 • Provide status monitoring of connected instruments and real-time plots STATEMENT OF NEED (SON) • Have the ability to send user notifications when a run is started, stopped or has an error • Allow registered users of the Connect platform to monitor any connected instrument • Allow users to run an assay plate without performing plate setup prior to beginning of run and to correct plate set up after the run • Allow export of all run results to excel and save graphs as .jpg files • Provide the ability to import a standard curve from one plate for analysis of another plate of the same instrument type • Allow for the selection of endogenous controls for gene expression experiments • Include statistical analysis tools including box-whisker plots, scatter plots and heat maps • Allow preselection of data to export and the location of the exported file • Allow for command-line functions for batch exporting of analyzed run files • Allow users to save predefined analysis settings for auto-exporting run data into format of choice • Contain online high-resolution melt application so user can specify number of clusters/variants and save defined controls and analysis setting.

4.1.6 Instrument Ecosystem The instrument ecosystem shall: • Be part of a desktop ecosystem that consists of a suite of qPCR applications. Instrument data can be grouped (multi-plate) for relative quantitation, SNP genotyping, standard curve, HRM analysis, and presence/absence analysis. • Be part of the Connect platform • Be able to connect to the Connect platform for run setup download and data uploads • Allow a run to be monitored by a web browser or mobile device • Allow batch-analysis of up to 500 experiments simultaneously through an online relative quantitation module • Provide an endogenous control selection tool for gene expression experiments in both the desktop and online quantitation software • Contain statistical anaylsis tools with both the desktop and online quantitation software • Contain a genotyping optimization tool that uses real-time data to pick the best cycle number for calling genotype in both the desktop and online genotyping software • Allow the user to specify number of clusters/variants and save defined controls and analysis settings with both desktop and online software.

4.1.7. Design 5 The system shall: • Have an application programming interface (API) included that allows programming routines to be developed for controlling the instrument • Be cUL, UL, CE, C-TICK, and WEEE compliant • Be developed and manufactured in a facility with quality system requirements that comply with ISO 13485 standards and ISO 9001 standards 4.1.8 Gene expression profiling • Users shall be able to select from more than 61,000 predesigned human, mouse and rat Applied BiosystemsTMTaqMan® Assays.

4.1.9 Able to accept the trade-in of Model STEPONEPLUS. Serial number 272008444 4.1.10 Must be compatible with protocols from Applied Biosystems StepOnePlus Real Time PCR (qPCR) allowing for consistency in experimental set-up 4.2 DELIVERY / INSTALLATION The contractor shall deliver and install the equipment within thirty (30) calendar days after receipt of award.