Cyanobacteria ddPCR and RT-ddPCR Laboratory Analysis

The contract requires the performance of digital droplet PCR and reverse transcription digital droplet PCR on cyanobacterial mat samples to detect the anaC gene, encompassing the full workflow from DNA and RNA extraction through molecular analysis and quality control. The work involves precise laboratory techniques to identify genetic markers associated with cyanobacterial activity, ensuring accurate detection and quantification under controlled conditions. All procedures must meet established standards for molecular diagnostics and sample integrity, with emphasis on reproducibility and data reliability. The solicitation is designated as a Small Business Set-Aside under NAICS code 541380, indicating it is reserved exclusively for small businesses, and is issued by the Department of the Interior’s Office of Acquisition Grants. Proposals must be submitted by June 30, 2026, at 12:00 PM, with the work expected to be performed at a location determined by the contractor. The contract type is a subcontract, and while specific performance location details are not provided, the scope demands specialized laboratory capabilities and expertise in advanced molecular biology techniques for environmental sample analysis.