Development of Inducible Point Mouse Line

This is a combined synopsis/solicitation for commercial items in accordance with the format in the Subpart 12.6, as supplemented with the additional information included in this notice. This announcement constitutes the only solicitation : proposals are being requested and a written solicitation will not be issued. This is not a small business set aside. This solicitation number is RFQ2800348 and a solicitation is issued as a request for quotation (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005- 63. The National Institute on Drug Abuse (NIDA), Station Support /Simplified Acquisition (SS/SA) Branch on behalf of the National Institute on Disorders and Stroke (NINDS) has a requirement for services. The Services will include the following: Bio-informatic analysis and cloning of the gene of interest: - Bioinformatic analysis, cloning and characterization of the gene of interest Construction of the targeting vector and designing of the screening strategy: - Design and construction of both the targeting vector and the positive control vector - Designing and setting up of the screening methodology ES cell transfection and homologous recombination: - Homologous recombination in ES cells and resistant clone isolation (400=400 c,ones) - Screening for homologous recombination (PCR and Southern Blot) ES Cell Blastocyct injection - ES cell blastocyst injection - Generation of chimeras Breeding to the F1 generation with wild type and FLP delete mice: - Breeding to the F1 generation and in vivo excision of the Neo cassette - Health monitoring of animals Quotations will be evaluated on the following criteria: Approach to the task Experience in development of inducible point mutant mouse lines Past Performance: Documented successful past performance of the stated services over the last 3 years. The anticipated period of performance is for one (1) year. Offerors will also be evaluated on best value in relation to price. Offerors must include a completed copy of the provision at 52.212-3, Offeror Representations and Certifications Commercial Items, with its offer, Clause 52.212-4, Contract Terms and Conditions Commercial Items, applies to this acquisition. Clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders Commercial Items, applies to this acquisition. The quotations are due December 19, 2012, 12:00PM EST and will be submitted electronically to simpsoi@mail.nih.gov, Idella Simpson, Contract Specialist, 31 Center Drive Room 1B59, Bethesda, MD 20892.