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This Government Contract opportunity from Department Of Agriculture was posted on April 26, 2023. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

Genome Sequencing Service (RNAseq)

Awarded
12405B23Q0226Federal

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Solicitation 12405B23Q0226 is a request for quotation issued by the USDA Agricultural Research Service for genome sequencing services. The selected provider will process 294 RNA samples from channel catfish tissues. Required services include mRNA isolation via polyA selection or rRNA removal, Illumina library preparation, and paired end 2x150bp sequencing. The contractor must provide at least 25 million read pairs per sample with a quality threshold of 80 percent of bases at Q30, delivering the final raw demultiplexed data in FASTQ format. Interested offerors must be registered in the System for Award Management and submit a signed SF18 offer page, a unique entity identifier, and a detailed quotation on company letterhead. The quotation must include unit pricing, total costs, and delivery fees, remaining valid for at least 60 days. All items are delivered FOB Destination to the Aquatic Animal Health Research Unit in Auburn, Alabama. Submissions were due by April 24, 2023, and all inquiries were managed electronically through the designated contracting officer.

General Info

Contract Value

$38,250

NAICS

541380 - Testing Laboratories and Services

Place of Performance

Auburn, AL, 36832, USA

Set-Aside

NONE

Award Issued Date

Documents

3

SF18+SOLICITATION+12405B23Q0226+AUBURN+AL.pdf

PDF

SF30+AMENDMENT+0001+SOLICITATION+12405B23Q0226.pdf

PDF

Statement+of+Work+Genome+Sequencing+Service+RNAseq.docx

DOCX

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Timeline

1 update
PhaseAwarded
Posted

Combined Synopsis

Notice updated

The notice description changed.

Awarded

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Submission Closed

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Organization & Contact Information

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AgencyDepartment Of Agriculture → USDA Ars Sea Aao Acq/per Prop
Contacts1 person available
OfficeSTONEVILLE, MS, 38776, USA
Office AddressSTONEVILLE, MS, 38776, USA
Contacts

Full Description

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This is a combined synopsis/solicitation for commercial items and commercial services prepared in accordance with the format in FAR subpart 12.6 and FAR Part 13, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. The solicitation number is 12405B23Q0226 and is issued as a request for quotation (RFQ). This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2023-02. The USDA, ARS, SEA, Aquatic Animal Health Research Unit (AAHRU), Auburn, Alabama location will provide 294 RNA samples. Auburn, Alabama location is seeking a service provider to: Perform mRNA isolation from total RNA using either polyA selection or rRNA removal. Perform library preparation for Illumina sequencing. Perform paired end, 2x150bp sequencing for each sample. Provide ?25M read pairs of sequencing information for each sample, with quality of ? 80% of bases ? Q30. Provide raw demultiplexed data in FASTQ format. The requirements for this solicitation are further detailed in the attached Statement of Work (SOW). Delivery Address: USDA, ARS, SEA, Aquatic Animal Health Research Unit (AAHRU) ATTENTION: Jason Abernathy 990 Wire Road Auburn, Alabama 36832 Quotation Submission. In order to be considered for award, Offerors shall respond by submitting: One (1) original fully completed and signed SF18 Offer page; acknowledgment of all amendments (if applicable). Provide SAM Unique Entity Identifier (UEI) number. Quotation (on company letterhead) detailing item description, unit price per item, total price, and delivery costs as well as any applicable duties, brokerage, or customs fees. Quotation must be valid for at least 60 days after receipt of quotation. Items delivered FOB Destination inclusive of all costs. Failure to demonstrate compliance will be cause to reject the quotation without further discussion. All responsible sources may submit an offer and will be considered. Questions. All questions pertaining to this solicitation must be in writing. All questions will be addressed by email only. Submit questions referencing solicitation number 12405B23Q0226 electronically via email to contact@example.gov. Questions are due on or before April 21, 2023, by 12:00 p.m. Central Time (CT). Telephone inquiries will not be accepted. Quotation Due Date. Quotations referencing solicitation number 12405B23Q0226 should be submitted electronically via email to contact@example.gov by Monday, April 24, 2023, no later than 3:00 p.m. Central Time (CT). No late quotations will be accepted. FAR clauses 52.212-1, 52.212-2, 52.212-3, 52.212-4 and 52.212-5 (and any addenda to those clauses) are included in the attached clauses and provisions document. Contractors intending to conduct business with the Federal Government MUST register with the System for Award Management (SAM) database prior to submitting a quotation. Registration is free and you may register online at www.sam.gov. Prospective contractors who have not completed electronic annual representations and certifications at http://www.sam.gov/ in conjunction with required registration in the System for Award Management (SAM) database, shall complete and submit FAR 52.212-3 Alternative 1 Offeror Representations and Certifications - Commercial Items and DFARS Representations and Certifications in SAM to be eligible to receive an award for this solicitation. Any amendments and documents related to this procurement will be available electronically at the Government Point of Entry (SAM.gov). Please continue to monitor this site for the release of any further amendments (if any) or other information pertaining to this solicitation. Amendment 0001 issued to provide answers to questions submitted by interested contractor. Q1. What is the sample type (ie cells, tissue blood, bacteria?) A1. Various tissues from channel catfish Q2. What is the species being sequenced? A2. Channel catfish (Ictalurus punctatus) Q3. Will samples be submitted in a single batch? A3. Yes Q4. Will there be quantity starting material of sample with minimum 200ng total RNA with concentration >10ng/ul, OD260>1.6 & RIN value >7 ? If not, what will be different or is unknown? A4. Expected, yes. We have not processed the samples yet

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