This Solicitation opportunity from Government of Canada was posted on November 25, 2024. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

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Genotyping-In-Thousands (GTseq) Genetic Marker Design for Four Aquatic Species

Closed

30006232International

Submission Closed11/25/2024, 01:00:00 PM UTC

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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NAICS: 541714

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POSTED

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DEADLINE

N/A

AI Contract Overview

Fisheries and Oceans Canada is seeking a contractor to develop, design, and validate 500 GTseq genetic markers to support the management of aquatic species. These markers will be used to produce repeatable genotypes for genetic analyses across four different species, including the Beluga and Inconnu, as well as two additional species to be identified. The markers must be specifically designed for genotyping on an Illumina MiSeq platform to provide high-quality genomic data for the Arctic, Ontario, and Prairie regions. The selected contractor will be responsible for the design, testing, and validation of the markers and will provide necessary support to optimize laboratory and data analysis setups at the Freshwater Institute. To facilitate this work, the Freshwater Institute will provide the required genomic data and DNA for testing. This initiative aims to enhance genotyping capabilities for researchers to promote sustainable aquatic ecosystems and economically prosperous maritime sectors.

General Info

Contractor will design and validate 500 GTseq genetic markers for four aquatic species.

Agency

Government of Canada → Fisheries and Oceans Canada

NAICS

541714 - Research and Development in Biotechnology (except Nanobiotechnology)

Place of Performance

CAN

Set-Aside

NONE

Documents

No documents available

No documents were posted with this contract.

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Uniform Contract Format

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Timeline

PhaseClosed

PostedNov 25

Solicitation

Response DeadlineNov 25

Deadline has passed

Submission Closed

11/25/2024, 01:00:00 PM UTC

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Organization & Contact Information

AgencyGovernment of Canada → Fisheries and Oceans Canada

Contacts1 person available

OfficeN/A

Organization / Agency

Government of Canada → Fisheries and Oceans Canada

Office AddressN/A

Contacts

Jackie LongmirePoint of Contact

Full Description

The Department of Fisheries and Oceans (DFO) has a requirement to develop, design and test 500 GTseq markers that will produce repeatable that can be used in genetic analyses related to management of aquatic species in Canada. The work will involve the following: To obtain a contract to develop, design and test 500 GTseq markers that will produce repeatable genotypes that can be used in genetic analyses related to management of aquatic species in Canada. To meet this objective, we would like to develop panels for four different species. Two of these species, Beluga (Delphinapterus leucas) and Inconnu (Stenodus leucichthys) have been identified as requiring this resource for management and two additional species that will be identified. Recent advances in next-generation sequencing (NGS) technology have provided methods to increase the proportion of the genome analyzed and allow neutral and adaptive diversity to be assessed. Once important regions of the genome are identified, it is possible to develop sets of single nucleotide polymorphisms (SNPs) through genotyping-in-thousands by sequencing (GTseq). With this method, many SNPs can be sequenced simultaneously by using multiplexed PCR primers in a consistent and reproducible manner. Therefore, the design, testing, and validation of GTseq genetic markers will ensure genotyping capabilities are available to researchers and will provide high quality genomic data to the Arctic and Ontario & Prairie regions to support Fisheries & Ocean’s mission to promote economically prosperous maritime sectors and fisheries, and sustainable aquatic ecosystems. Design, testing, and validation of 500 GTseq genetic markers to conduct genetic studies on up to four different aquatic species. GTseq genetic markers Must be designed for genotyping samples on an Illumina MiSeq. The Contractor will also provide support, as needed, to assist in further optimizing laboratory and data analysis set up for genotyping the developed markers in the genetics and genomics laboratory at the Freshwater Institute. The Freshwater Institute will provide the Contractor genomic data and DNA suitable for marker testing and validation.