This Combined Synopsis/Solicitation opportunity was posted on September 8, 2016. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

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Immunosenescence and Inflammatin Associated with Aging

Awarded

HHS-NIH-NIDA-SSSA-2016-RFQ-543Federal

Submission Closed09/12/2016, 05:00:00 PM UTC

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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NAICS: 541380

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Solicitation # 121589

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Utility Services Department

POSTED

about 16 hours ago

DEADLINE

in about 1 month

AI Contract Overview

This procurement order, titled Immunosenescence and Inflammation Associated with Aging, seeks a contractor to perform deep sequencing of RNA and chromatin immunoprecipitation for cell subsets derived from human peripheral blood mononuclear cells. The project focuses on examining age-associated changes in gene expression and epigenetic states across ten cell subsets from 30 volunteers of varying ages. This collaborative effort involves several NIA/IRP laboratories and utilizes data from the BLSA, GESTALT, and normal donor projects. The scope of work includes measuring chromatin accessibility changes and conformational changes using ChIA-Pet. While the NIA will handle the collection of cytapheresis packs, cell fractionation, and the preparation of ATAC and ChIA-Pet libraries, the contractor will be responsible for the deep sequencing of the provided purified RNA, DNA, and libraries. The performance of this contract is located in Baltimore, Maryland.

General Info

Agency

N/A

Contract Value

$126,000

NAICS

541380 - Testing Laboratories and Services

Place of Performance

733 North Broadway, Room 351, BALTIMORE, MD, 21205, USA

Set-Aside

NONE

Awardee

THE JOHNS HOPKINS UNIVERSITY

Award Issued Date

09/23/2016, 12:00:00 AM UTC

Documents

No documents available

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Timeline

PhaseAwarded

PostedSep 8

Combined Synopsis

AwardedSep 23

Contract was awarded

Submission Closed

09/12/2016, 05:00:00 PM UTC

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Organization & Contact Information

AgencyN/A

Contacts2 people available

OfficeN/A

Organization / Agency

N/A

Office AddressN/A

Contacts

Danielle R. BrownContract Specialist

contact@example.gov

+1 (555) 000-0000

Rachelle TriceSupervisory Contract Specialist

contact@example.gov

+1 (555) 000-0000

Full Description

The requested procurement order is to contract deep sequencing of RNA and chromatin immunoprecipipitation (chip) cell subsets obtained from human peripheral blood mononuclear cells from volunteers for ongoing projects (BLSA, GESTALT, normal donors). Additionally, deep sequencing will be carried out to measure chromatin accessibility changes with age and chromatin changes conformational changes (ChIA-Pet). The proposed project will examine age-associated changes in gene expression and epigenetic state of 10 cell subsets purified from human peripheral blood. This is a collaborative venture that involves several NIA/IRP laboratories, including, LMBI, TGB, LMG, and LG. Cytapheresis packs will be collected from 30 volunteers of varying age-groups and cells fractioned by a combination of magnetic bead purification and flow cytometry. These cells will be used to isolate RNA and carry out ChIP and ATAC. Purified RNA and DNA will be provided to Dr. Hao for deep sequencing. Additionally, ChIA-Pet will be used to obtain chromatin structural information. ATAC and ChIA-Pet libraries will be prepared at the NIA and provided to the contractor.