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This Government Contract opportunity from Department Of Agriculture was posted on March 31, 2021. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

Pacbio Sequencing of 8 citrus (Pomelo) tissue samples

Awarded
12405B21P0080Federal

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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NAICS: 541380
New
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California Department of General Services

POSTED

about 10 hours ago

DEADLINE

in 26 days
NAICS: 541380
New
SLED
Invitation For Bid P2675010, Analytical Testing Services
Solicitation # P2675010
The California Department of Fish and Wildlife (CDFW) is seeking a contractor to provide analytical testing services to support the Office of Spill Prevention Response Laboratory Program. The primary objective is to establish contingency capacity for water quality and nutrient analysis, ensuring continuity of services during catastrophic instrument failures or providing testing capabilities not available in-house. These services support research studies with partner state agencies and enforcement actions aimed at protecting state waters and identifying pollutants that impact wildlife. The contract has a maximum value of 31,000.00 dollars, and the award will be granted to the lowest responsive and responsible bidder. Eligible contractors must possess California Environmental Laboratory Accreditation Program (ELAP) certification, particularly for wastewater and hazardous waste testing. Bidders are required to provide a Quality Assurance Manual, Method Detection Limits, and reporting limits for all parameters. The submission process requires several certifications, including the Darfur Contracting Act and the Bidder Declaration Form, with a deadline of November 16, 2026. Preferences and incentives are available for certified Small Businesses and Disabled Veteran Business Enterprises (DVBE), including a 5% bidding preference for small businesses and specific incentive rates for DVBE participation. The contractor is responsible for providing all pre-cleaned sample containers and preservatives, with these costs included in the quoted price.
California Department of Fish & Wildlife

POSTED

about 10 hours ago

DEADLINE

in 19 days

AI Contract Overview

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This award covers a genomics project for the Pacbio sequencing of eight citrus Pomelo tissue samples, which must be processed together as a single batch. The scope of work includes the extraction of high molecular weight DNA using a modified CTAB method, the construction of HiFi libraries size-selected between 15-25kb, and sequencing on a Pacbio Sequel II system. Each sample will utilize one SMRT cell with SeqII v2.0 chemistry, with an expected yield of 16-24Gb of QV20+ CCS data per cell, although specific yields are not guaranteed. The project includes CCS analysis of all raw data and the delivery of summarized statistics and outputs via secure FTP or cloud storage, though no additional bioinformatics or assembly services are provided. The total cost encompasses all materials, supplies, labor, and fees for the eight data sets, while the customer remains responsible for all shipping costs. The contract is managed by the USDA Agricultural Research Service with performance taking place in Tucson, Arizona.

General Info

Contract Value

$37,200

NAICS

541380 - Testing Laboratories and Services

Place of Performance

Tucson, AZ, 85719, USA

Set-Aside

NONE

Award Issued Date

Documents

0

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Timeline

PhaseAwarded
Posted

Award Notice

Awarded

Contract was awarded

Submission Closed

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Organization & Contact Information

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AgencyDepartment Of Agriculture → USDA Ars Sea Aao Acq/per Prop
Contacts1 person available
OfficeSTONEVILLE, MS, 38776, USA
Office AddressSTONEVILLE, MS, 38776, USA
Contacts

Full Description

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This award is for a Genomics Project of Pacbio Sequencing of 8 citrus (Pomelo) tissue samples that must run together as a batch of 8. Receive 8 tissue samples and extract HMW DNA suitable for Pacbio sequencing a- Must receive correct amount of tissue and quality of tissue for HMW DNA; b- Extractions performed using modified CTAB; QC data available c- cost for shipping is the responsibility of the customer Produce Pacbio HiFi sequence libraries a- Construct one HiFi library from each DNA sample b- HiFi library, size selected 15-25kb c- Amnt of library correct for Number of cells to run Sequence libraries on Pacbio Sequel II; FOR EACH SAMPLE: a- Sequencing Chemistry used is SeqII v2.0; SMRT cells are 8M v1 b- Sequence 1 cell HiFi CCS (30hr) c- Each CCS cell is expected to produce 16-24Gb of QV20+ CCS data. No guarantee on yield. Run CCS analysis on all raw data; summarize data and deliver via secure FTP a- CCS analysis run on each HiFi CCS cell raw data; b- No additional bioinformatics. No Assembly c- AGI QC data, monitor progress, summarize outputs and stats. Deliver CCS data via secure FTP (or cloud). TOTAL COST for 8 sample data sets (from 8 cells sequenced) Includes all materials, supplies, labor, and fees

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