Pacbio Sequencing of 8 citrus (Pomelo) tissue samples

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested, and a written solicitation will not be issued. The solicitation number is 12405B21Q0030 and is issued as a request for quotation (RFQ). The NAICS code is 541380, Testing Laboratories. This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2021-04. The USDA, ARS, Horticultural Research Laboratory in Ft. Pierce, FL requires the following service: Genomics Project of Pacbio Sequencing of 8 citrus (Pomelo) tissue samples that must run together as a batch of 8. Receive 8 tissue samples and extract HMW DNA suitable for Pacbio sequencing a- Must receive correct amount of tissue and quality of tissue for HMW DNA; b- Extractions performed using modified CTAB; QC data available c- cost for shipping is the responsibility of the customer Produce Pacbio HiFi sequence libraries a- Construct one HiFi library from each DNA sample b- HiFi library, size selected 15-25kb c- Amnt of library correct for Number of cells to run Sequence libraries on Pacbio Sequel II; FOR EACH SAMPLE: a- Sequencing Chemistry used is SeqII v2.0; SMRT cells are 8M v1 b- Sequence 1 cell HiFi CCS (30hr) c- Each CCS cell is expected to produce 16-24Gb of QV20+ CCS data. No guarantee on yield. Run CCS analysis on all raw data; summarize data and deliver via secure FTP a- CCS analysis run on each HiFi CCS cell raw data; b- No additional bioinformatics. No Assembly c- AGI QC data, monitor progress, summarize outputs and stats. Deliver CCS data via secure FTP (or cloud). TOTAL COST for 8 sample data sets (from 8 cells sequenced) Includes all materials, supplies, labor, and fees Any quotes not meeting the minimum specifications will not be considered. DOCUMENTS TO BE INCLUDED IN QUOTE: In order to be considered for award Offerors shall provide 1) a Quote on company letterhead detailing the item description, unit price per item, discounts for trade-ins, total price and valid for at least 60 days after receipt of quote; and 2) FAR 52.212-3, OFFEROR REPRESENTATIONS AND CERTIFICATION-COMMERCIAL ITEMS; The Representation and Certification section must be completed electronically in the System for Award Management (SAM) website at www.sam.gov. REJECTION OF QUOTATION: Failure to demonstrate compliance will be caused to reject the quote without further discussions. All responsible sources may submit an offer and will be considered. The Service Contract Act Wage Determinations will apply to this contract. The following clauses and provisions apply to this acquisition. 52.252-2 Clauses Incorporated by Reference; 52.212-4 CONTRACT TERMS AND CONDITIONS - COMMERCIAL ITEMS; 52.212-5 CONTRACT TERMS AND CONDITIONS REQUIRED TO IMPLEMENT STATUTES OR EXECUTIVE ORDERS - COMMERCIAL ITEMS applies,; 52.222-3, Convict Labor; 52.233-3, Protest after award; 52.222-26, Equal Opportunity; 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans; 52.222-36, Affirmative Action for Workers with Disabilities; 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans; 52.222-19, Child Labor - Cooperation with Authorities and Remedies; 52.223-2 Affirmative Procurement of Biobased Products Under Service and Construction Contracts; 52.223-15 Energy Efficiency in Energy-Consuming Products; 52.223-17 Affirmative Procurement of EPA-designated Items in Service and Construction Contracts; 52.225-13, Restrictions on Certain Foreign Purchases; 52.232-33, Payment by Electronic Funds Transfer - System for Award Management; applicable to this acquisition; 52.252-1 Solicitation Provisions Incorporated by Reference; 52.212-1 INSTRUCTIONS TO OFFERORS - COMMERCIAL ITEMS; 52.223-1 Biobased Product Certification; 52.223-4 Recovered Materials Certification; 52.212-2 Evaluation-Commercial Items ; 52.212-3 OFFEROR REPRESENTATIONS AND CERTIFICATIONS - COMMERCIAL ITEMS; 52.217-8 Option to Extend Services. QUOTED PRICE MUST INCLUDE: any delivery costs as well as any applicable duties, brokerage, or customs fees. DELIVERY DUE DATE: The Government requires delivery on or before 30 ARO, however, each offeror shall include their proposed delivery schedule as part of their quotation. PAYMENT: Any award made under this solicitation is subject to 31 CFR Part 208 which requires all payments made by the government to be made by electronic funds transfer. Contractors intending to conduct business with the Federal Government MUST register with the System for Award Management (SAM) database prior to submitting quotation. You may register online at www.sam.gov. Any amendment and documents related to this procurement will be available electronically at the Internet site: http://www.fbo.gov. FAR 52.212-2, Evaluation-Commercial Item, the significant evaluation factors, in the relative order of importance, are (i) technical capability of the item offered to meet the Government requirement including delivery date; (ii) past performance; and (iii) price (based on F.O.B. Destination), technical and past performance are more important than price. Award will be made based on overall BEST VALUE to the Government. The government anticipates award of a firm fixed priced contract resulting from this solicitation to the responsible offeror whose offer conforms to the specifications that will be the most advantageous to the government in accordance with FAR 13.106-2. Responses shall include technical specifications, descriptive material and capabilities of the offeror's equipment. Offerors shall identity where the offered item meets or does not meet each of the Government's functional and performance minimum specifications listed herein. All sources wishing to furnish a quotation must respond by 2:00 p.m. central standard time, February 19, 2021. Quotations are to be addressed to Jessica Pacheco, Contract Specialist, at contact@example.gov. Additional information may be obtained by contacting the Contract Specialist by email.