single cell RNASEQ for iPSC

INTRODUCTION THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME). The National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Office of Acquisitions (OA) on behalf of the on behalf of the National Institute of Digestive, Diabetes & Kidney Diseases intends to award a purchase order without providing for full and open competition (Including brand-name) to SEQMATIC, LLC for the purchase of single cell RNASEQ for iPSC. NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE The intended procurement is classified under NAICS code 541380 with a Size Standard of $16.50. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2021-02 effective November 23, 2020. This acquisition is conducted under the procedures as prescribed in FAR subpart 13'Simplified Acquisition Procedures at an amount not exceeding the simplified acquisition threshold ($250,000). STATUTORY AUTHORITY This acquisition is conducted under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302- FAR 6.302-1'Only one responsible source and no other supplies or services will satisfy agency requirements 41 U.S.C. 253(c)(1). PERIOD OF PERFORMANCE Within 90 Days of Award Place of Performance National Institutes of Health National Institute of Digestive, Diabetes & Kidney Diseases Genomics Building 445 N. 5th Street Phoenix, AZ 85004 ? DESCRIPTION OF REQUIREMENT The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) conducts and supports research on many of the most serious diseases affecting public health. The Institute supports much of the clinical research on the diseases of internal medicine and related subspecialty fields, as well as many basic science disciplines. The Institute's Division of Intramural Research encompasses the broad spectrum of metabolic diseases such as diabetes, obesity, inborn errors of metabolism, endocrine disorders, mineral metabolism, digestive and liver diseases, nutrition, urology and renal disease, and hematology. Basic research studies include biochemistry, biophysics, nutrition, pathology, histochemistry, bioorganic chemistry, physical chemistry, chemical and molecular biology, and pharmacology. NIDDK extramural research is organized into 4 divisions: Diabetes, Endocrinology, and Metabolic Diseases; Digestive Diseases and Nutrition; Kidney, Urologic, and Hematologic Diseases; and Extramural Activities. The Institute supports basic and clinical research through investigator-initiated grants, program project and center grants, and career development and training awards. The Institute also supports research and development projects and large-scale clinical trials through contracts. _________________________ The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to obtain single cell RNAseq for more samples. More sequencing is required to analyze more time points during iPSC development. The goal of the project is to map the entire transcriptome including very rare transcripts. The total RNA sample is from three different iPSC lines (stem cells) during different stages of differentiation. PURPOSE AND OBJECTIVES To compare these different stages and detect very rare transcripts, RNASEQ paired end with 50bp reads is needed. Data analysis is to accompany these single cell RNAseq assays. OBJECTIVE The objective is to purchase single cell RNASEQ for iPSC. SALIENT / REQUIRED FEATURES AND SPECIFICATIONS Specifically, the NIDDK requires the following: TASKS AREAS Independently, and not as an agent of the Government, the contractor shall perform all of the detailed Task Areas listed below: Task Area 1 - We will provide single cell samples (quantified in our lab by bioanalyzer at a concentration of 1 - g or more for single cell RNAseq and library construction. Task Area 2 - Deliverables: - Data delivered via S3 cloud. - Raw sequencing data in FASTQ format (generated via cellranger mkfastq) - Cellranger count: 1. BAM files 2. Count summary (html and csv) 3. Loupe cell browser file CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME) DETERMINATION The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted as prescribed in FAR Part 10'Market Research. Only one source is available: Per FAR 13.106-1(b)(1) the Contracting Officer has determined SEQMATIC, LLC to be the only reasonable available source to provide single cell RNASEQ for iPSC. This acquisition was pursued on a sole source basis centered on the NIDDK having initiated a collaboration with SEQMATIC, LLC previously we need to now scale-up based on the results of that earlier project. The NIDDK must continue using this vendor in the project for scientific continuity. That continuity will help the NIDDK to avoid the introduction of new variables into its research and to avoid long delays and additional costs resulting from retesting products from other potential suppliers. Failure to procure these deliverables from SEQMATIC, LLC would result in a significant loss of research. SEQMATIC, LLC has been able to work with degraded samples and has communicated well when samples are beyond hope. SeqMatic will work on the same platform that are other samples have been processed for continuity of data results. CLOSING STATEMENT This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties bona-fide capabilities for fulfilling the requirement and include: descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all the foregoing requirements, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement. All responses to this notice shall be submitted electronically by 9:00 am Eastern Standard Time, on Tuesday, January 12, 2021 to the Contract Specialist, Amber Harris, at contact@example.gov. Assessment of Capability Lowest Price Technically Acceptable