66--This requirement is for development of monoclonal antibodies to tetrodotoxin five (5) stages in support of the Edgewood Chemical Biological Center (ECBC), Aberdeen Proving Ground, Maryland.

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 05-82. The combined synopsis/solicitation number is W91ZLK-15-T-0236 used as a request for proposal (RFP). The requirement is under the associated North America Industry Classification System (NAICS) Code is 325413 In-Vitro Diagnostic Substance Manufacturing and the Business Size Standard is 500. The Government contemplates award of a Firm-Fixed Price contract, Lowest Price Technically Acceptable (LPTA) under FAR Part 12, Acquisition of Commercial Items. The U.S. Army Contracting Command, Aberdeen Installation Contracting Division, Aberdeen Proving Ground, Maryland intends to issue a Competitive quote mark Small Business quote mark award. This requirement is a Total Small Business Set Aside. This requirement is not being procured in accordance with FAR Part 8 and only Open Market, NOT General Service Administration GSA quotations are being accepted from responsible contractors. NOTE: TO Offerors: Offers shall note the lead time or approximate delivery date for items offered. This requirement includes a twelve (12) month base period and two (2) twelve (12) month option periods. Acceptance and inspection shall be at Destination. Final completion/destination shall be at US Army Edgewood Chemical Biological Center (ECBC), Aberdeen Proving Ground, Maryland 21010. Description of the Requirements: This requirement is for development of monoclonal antibodies to tetrodotoxin five (5) stages in support of the Edgewood Chemical Biological Center (ECBC), Aberdeen Proving Ground, Maryland. Identification of some analytics is currently hindered by the lack of a low cost, sensitive detection method. Monoclonal antibodies are often useful tools for the development of new analytical method. Monoclonal antibodies are often useful tools for the development of new analytical methods. The government is seeking all personnel, equipment, tools, materials, supervision, and non-personal services necessary to support Environmental Biological Monitory Laboratory as defined in CLINS (contract line items) and Description. The services to be conducted by the contractor are to include five stages: CLINS and Description: (Also, see Attachment #1) CLIN 0001: Stage 1 Immunogen Synthesis: Development of monoclonal antibodies to tetrodotoxin (5 stages). Covalent attachment of tetrodotoxin (provided by client) to carrier proteins will be performed. The immunogen will be made using Bovine Serum Albumin and/or Proprietary Protein as the carrier protein CLIN 0002: Stage 2 HRP-Tetrodotoxin Synthesis: Development of monoclonal antibodies to tetrodotoxin (5 stages). The compound (or an analog) will be conjugated to HorseRadish Peroxidase. This Tetrodotoxin-HRP conjugate will be used to screen antibodies for recognition and affinity of clones for the toxin. CLIN 0003: Stage 3 Immunization: Development of monoclonal antibodies to tetrodotoxin (5 stages). Groups of nine (9) mice will be immunized using each immunogen(s) synthesized in stage one. Mice sera will be screened for recognition of Tetrodotoxin-HRP one week after the first boost (one month after first immunization). Booster injections and sera screens will continue for an additional two months (three months total). Completion of this stage will be demonstration of sera titers showing recognition of the peptide OR three months of immunizations. CLIN 0004: Stage 4 Fusion: Development of monoclonal antibodies to tetrodotoxin (5 stages) A fusion will be performed on two (2) mice demonstrating the highest titer response. Stage Four will take not more than 1 month. Completion of this stage will be the identification of at least four hybridomas demonstrating a dose response to tetrodotoxin CLIN 0005: Stage 5 Cloning: Development of monoclonal antibodies to tetrodotoxin (5 stages). The fifth stage of this project will be comprised of cloning the stable cell lines identified in stage four. This step will take not more than 2 months. The four clones with the best sensitivity to tetrodotoxin in a dose response assay will be isolated and grown to confluence resulting in 40 mL of supernatant containing maximally expressed monoclonal antibody. This supernatant will be delivered to the customer for evaluation. Note: four clones will be delivered to allow for comparison of sensitivity, specificity and possible matrix interferences in assay development. NO TELEPHONE INQUIRES WILL BE HONORED Instructions to Offerors: Offerors shall submit price proposals in accordance with the CLIN structure above and Attachment #1. Partial quotes will not be evaluated by the Government. Award will be made on the basis of the lowest evaluated price proposal meeting or exceeding the acceptability standards for non-cost factors in accordance with FAR 15.101-2. 52.212-1 Instruction to Offerors Commercial Items/Services This clause applies in its entirety and there are currently no addenda to the provision. ALL PROPOSALS SHALL BE SUBMITTED VIA EMAIL WITH THE SUBJECT LINE: W91ZLK-15-T-0236 PROPOSAL FROM (INSERT COMPANY NAME) (SEE ATTACHMENT #1) Proposal Format: Detailed Price Proposal which address all CLINS 1. Header Page Company Name, DUNS, CAGE, Business Type/Size, ETN# and point of contact information to include: Name, title, email address, and telephone number including extension. 52.212-2, Evaluation Commercial Items The contract will be awarded to the Lowest Price Technically Acceptable (LPTA) offeror. The technical evaluation will be a determination based on information furnished by the vendor in the submitted proposals. The Government is not responsible for locating or securing any information which is not identified in the offer. The Government reserves the right to make an award without discussions. Proposals will be evaluated by adding all Firm-Fixed Price costs for Items 0001 thru 0005. 52.212-3 Offeror Representations and Certifications -- Commercial Items. All quotations from responsible sources will be fully considered. Vendors, who are not registered in the System for Award Management (SAM), prior to award, will not be considered. Vendors may register with SAM by going to www.sam.gov. 52.212-4 Contract Terms and Conditions Commercial Items This clause applies in its entirety and there are currently no addenda to the provision. 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items. This clause applies, and the following clauses are incorporated by reference. The following provisions and clauses will be incorporated by reference, and the full text of the references may be accessed electronically at this address: http://farsite.hill.af.mil: 52.222-50, Combating Trafficking in Persons (FEB 2009) (22 U.S.C. 7104(g)). Alternate I (Aug 2007) of 52.222-50 (22 U.S.C. 7104(g)). 52.233-3, Protest After Award (AUG 1996) (31 U.S.C. 3553). 52.233-4, Applicable Law for Breach of Contract Claim (OCT 2004) (Pub. L. 108-77, 108-78). 52.204-10, Reporting Executive Compensation and First-Tier Subcontract Awards (July 2013) (Pub. L. 109-282) (31 U.S.C. 6101 note). 52.209-6, Protecting the Government's Interest When Subcontracting with Contractors Debarred, Suspended, or Proposed for Debarment. (July 2013) (31 U.S.C. 6101 note). 52.209-9, Updates of Publicly Available Information Regarding Responsibility Matters (July 2013) (41 U.S.C. 2313). 52.209-10, Prohibition on Contracting with Inverted Domestic Corporations (MAY 2012) (section 738 of Division C of Pub. L. 112-74, section 740 of Division C of Pub. L. 111-117, section 743 of Division D of Pub. L. 111-8, and section 745 of Division D of Pub. L. 110-161). 52.217-8 Option to Extend Services (Nov 1999) The Government may require continued performance of any services within the limits and at the rates specified in the contract. These rates may be adjusted only as a result of revisions to prevailing labor rates provided by the Secretary of Labor. The option provision may be exercised more than once, but the total extension of performance hereunder shall not exceed 6 months. The Contracting Officer may exercise the option by written notice to the Contractor at least 30 days before the contract expires. 52.219-6, Notice of Total Small Business Set-Aside (NOV 2011) (15 U.S.C. 644). 52.219-28, Post Award Small Business Program Representation (July 2013) (15 U.S.C. 632(a)(2)). 52.222-3, Convict Labor (June 2003) (E.O. 11755). 52.222-35, Equal Opportunity for Veterans (Sep 2010)(38 U.S.C. 4212). 52.222-36, Affirmative Action for Workers with Disabilities (Oct 2010) (29 U.S.C. 793). 52.222-37, Employment Reports on Veterans (Sep 2010) (38 U.S.C. 4212). 52.223-18, Encouraging Contractor Policies to Ban Text Messaging While Driving (Aug 2011) (E.O. 13513). 52.225-13, Restrictions on Certain Foreign Purchases (June 2008) (E.O.'s, proclamations, and statutes administered by the Office of Foreign Assets Control of the Department of the Treasury). 52.232-33, Payment by Electronic Funds Transfer-System for Award Management (July 2013) (31 U.S.C. 3332). 52.222-41, Service Contract Act of 1965 (Nov 2007) (41 U.S.C. 351, et seq.). 52.222-42, Statement of Equivalent Rates for Federal Hires (May 1989) (29 U.S.C. 206 and 41 U.S.C. 351, et seq.). 52.222-43, Fair Labor Standards Act and Service Contract Act-Price Adjustment (Multiple Year and Option Contracts) (Sep 2009) (29 U.S.C. 206 and 41 U.S.C. 351, et seq.). 52.222-17, Nondisplacement of Qualified Workers (JAN 2013) (E.O.13495). Notwithstanding the requirements of the clauses in paragraphs (a), (b), (c), and (d) of this clause, the Contractor is not required to flow down any FAR clause, other than those in this paragraph (e)(1)in a subcontract for commercial items. Unless otherwise indicated below, the extent of the flow down shall be as required by the clause- (i) 52.203-13, Contractor Code of Business Ethics and Conduct (APR 2010) (Pub. L. 110-252, Title VI, Chapter 1 (41 U.S.C. 251 note). (ii) 52.219-8, Utilization of Small Business Concerns (Jul 2013) (15 U.S.C. 637(d)(2) and (3)), in all subcontracts that offer further subcontracting opportunities. If the subcontract (except subcontracts to small business concerns) exceeds $650,000 ($1.5 million for construction of any public facility), the subcontractor must include 52.219-8 in lower tier subcontracts that offer subcontracting opportunities. (iii) 52.222-17, Nondisplacement of Qualified Workers (JAN 2013) (E.O. 13495). Flow down required in accordance with paragraph (l) of FAR clause 52.222-17. (iv) 52.222-26, Equal Opportunity (MAR 2007) (E.O. 11246). (v) 52.222-35, Equal Opportunity for Veterans (SEP 2010) (38 U.S.C. 4212). (vi) 52.222-36, Affirmative Action for Workers with Disabilities (OCT 1998) (29 U.S.C. 793). (vii) 52.222-40, Notification of Employee Rights Under the National Labor Relations Act (DEC 2010) (E.O. 13496). Flow down required in accordance with paragraph (f) of FAR clause 52.222-40. (viii) 52.222-41, Service Contract Act of 1965 (Nov 2007) (41 U.S.C. 351, et seq.). (ix) 52.222-50, Combating Trafficking in Persons (FEB 2009) (22 U.S.C. 7104(g)). Alternate I (AUG 2007) of 52.222-50 (22 U.S.C. 7104(g)). (x) 52.222-51, Exemption from Application of the Service Contract Act to Contracts for Maintenance, Calibration, or Repair of Certain Equipment--Requirements (Nov 2007) (41 U.S.C. 351, et seq.). (xi) 52.222-53, Exemption from Application of the Service Contract Act to Contracts for Certain Services-- Requirements (FEB 2009) (41 U.S.C. 351, et seq.). (xii) 52.222-54, Employment Eligibility Verification (JUL 2012). (xiii) 52.225-26, Contractors Performing Private Security Functions Outside the United States (Jul 2013) (Section 862, as amended, of the National Defense Authorization Act for Fiscal Year 2008; 10 U.S.C. 2302 Note). (xiv) 52.226-6, Promoting Excess Food Donation to Nonprofit Organizations. (MAR 2009) (Pub. L. 110-247). Flow down required in accordance with paragraph (e) of FAR clause 52.226-6. (xv) 52.247-64, Preference for Privately Owned U.S.-Flag Commercial Vessels (FEB 2006) (46 U.S.C. Appx 1241 (b) and 10 U.S.C. 2631). Flow down required in accordance with paragraph (d) of FAR clause 52.247-64. While not required, the contractor May include in its subcontracts for commercial items a minimal number of additional clauses necessary to satisfy its contractual obligations. 52.217-8 Option to Extend Services (Nov 1999) The Government may require continued performance of any services within the limits and at the rates specified in the contract. These rates may be adjusted only as a result of revisions to prevailing labor rates provided by the Secretary of Labor. The option provision may be exercised more than once, but the total extension of performance hereunder shall not exceed 6 months. The Contracting Officer may exercise the option by written notice to the Contractor at least 30 days before the contract expires. The full text Federal Acquisition Regulation (FAR) and Defense Federal Acquisition Regulation (DFAR) references may be accessed electronically at this address: http://farsite.hill.af.mil . All questions must be submitted via email to contact@example.gov no later than 16 July 2015, 3:00 p.m. EDT. SUBJECT LINE: W91ZLK-15-T-0236 Questions from (INSERT COMPANY NAME). All questions will be answered via an amendment to the solicitation on FBO. NO TELEPHONE INQUIRES WILL BE HONORED. Proposals must be signed, dated, and received no later than 24 July 2015, 3:00 p.m. DST via email to contact@example.gov. NO TELEPHONE INQUIRES WILL BE HONORED