--AKTAPURIFIER UPC 100W/DESKTOP with accessories.

Liquid Chromatography System for Protein Purification We require the ability to purify biomolecules from complex starting materials. The system we will use must be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. We require a flow rate range of .01-100 mls/min at pressures ranging from 0-10 MPa (1450 psi). Because the resolution we require may necessitate the use of very precise, shallow gradients, the system must be a two-pump gradient system, with dynamic mixing, as evidence suggests that this configuration produces the highest accuracy and precision in gradient formation. Because we will be using salt buffers, the entire wetted flow path of the system must be inert to halides. The pump heads must be titanium with valves and tubing in PEEK. The system pumps should allow operation of the system at zero backpressure to ensure system compatibility with standard low-pressure chromatography supports often used in affinity chromatography and other techniques. The controller must allow programs to be time-based, volume-based, or column-volume based. The system should include the option of a fraction collector that is fully integrated into the system and fully controllable via the system control software. This fraction collector must have the capacity to fractionate into 175 x 12-mm diameter tubes or 95 x 10-18mm tubes. The system must include a motorized valve for sample injection. In order to facilitate cleaning, storage, selection of our various chromatographic columns, and further automation the system must have the ability to control 8 more motorized valves for a total 9 motorized valves. This is necessary for buffer selection, injection, flow diversion, diverting peaks to sample loops etc. Also, these motorized valves should provide feedback to the control system to ensure proper orientation of all valves, and documentation of valve positions at all times. The valves should be driven electronically and require no external air or pressure to operate. The system controller must allow the user to be entering details of the next purification at the same time the current separation is being done, or to simultaneously be integrating and evaluating the results of earlier runs. The software must allow complete manual control of all parameters and running conditions while running a method. Control of motorized valves, fraction collector, dynamic gradient mixer, and autosampler must all be possible from the system controller. The pumps, UV, pH and conductivity monitors, valves and fraction collector must all be specified for use in a cold room at 4 degrees C. The controller must accept seven input signals (e.g. UV wavelength, conductivity, pH, temperature, pressure). It must be capable of plotting, and integrating these signals, as well as using them to trigger sub-routines in the programming when the signal exceeds a preset threshold, to allow the automation of multi-dimensional separations. The system must include one automatic three-position injection valve (load, inject, and wash positions), which will allow for sample loading onto the column. The system controller should allow for automation of the optimization of separation parameters (e.g. sample volume, flow rate, gradient slope). This should allow a small amount of sample to be consumed in optimizing the purification scheme, after which the best regime can be scaled up on the same LC system. Column equilibration before and after sample injection and elution must be capable of being monitored, with a feed-back loop to the control system, so we can be certain equilibration is completed, without consuming excess volume of sometimes expensive buffers. Documentation of each run should be automatic and exhaustive, so that for purposes of subsequent replication and eventual publication, no detail is left unrecorded. Access to the controller must be by user name with password and level of access by each user must be definable by administrator. The software must be able to operate on the Windows XP operating system. Post-run chromatographic analysis and reporting functions should also be included. In order to protect our columns, the system must have a definable pressure limit system defined when the column is selected from a list which will stop the pump and generate an audible and visual warning, in the event pressure exceeds the preset limit. We also require that the controller allow the user the option of including in his/her chromatographic methods programs, commands which will automatically reduce the flow rate if an over-pressurization occurs as sample is being applied or eluted from the column. Additional variables which should also be programmable to trigger sub-routines (e.g. new tube on fraction collector; valve changes; etc.) should include UV or conductivity signals. The controller should permit automatic running of up to 99 different chromatographic programs in a user-designated sequence. It must turn off the pump flow when a signal is received from the fraction collector indicating there are no tubes remaining, so that valuable sample is preserved. The system must automatically and continuously be able to record throughout each chromatographic run the following parameters: flow rate; UV signal; actual AND programmed conductivity of the eluent; eluent pH; system pressure; accumulated time of run; all valve positions and changes in position; activity of a fraction collector - and it must allow these recorded data to be called up later for interpretation and to aid in future replication of results. It must allow for post-run analysis of chromatograms, export of data to other spreadsheet programs, and generation of written reports. Set-up and training must be provided by the vendor, at no additional cost.