This Pre-Solicitation opportunity was posted on May 21, 2004. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

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B -- Sequencing of PCR Amplicons

Closed

Reference-Number-PSM-511-04Federal

Submission Closed06/10/2004, 05:00:00 AM UTC

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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AI Contract Overview

The USDA Agricultural Research Service in Albany, California, is seeking a vendor to provide bi-directional sequencing of PCR amplicons. The selected vendor will receive pre-performed PCR reactions and must utilize a fully automated production facility equipped with an integrated laboratory information management system to track sample quality and genealogy in real time. Technical requirements specify the use of an ABI 3730 for detection to maintain data consistency with existing projects, with a required pass rate of 85 to 90 percent and average PHRED 20 read lengths between 650 and 700 base pairs. To ensure high linkage rates, forward and reverse sequencing reads must be processed on the same plate. Deliverables include chromatograms, PHRED quality scores, and FASTA sequence files transmitted via FTP. The contract stipulates a turnaround time of 10 business days for batches of 10,000 amplicons or fewer. This presolicitation, identified by reference number PSM-511-04, was posted on May 21, 2004, with a response deadline of June 10, 2004.

General Info

USDA seeks vendor for automated bi-directional PCR amplicon sequencing with specific technical standards.

Agency

N/A

NAICS

541380 - Testing Laboratories and Services

Place of Performance

USDA, Agricultural Research ServiceWestern Regional Research Center Location Support Services 800 Buchanan Street Albany, Calif

Set-Aside

NONE

Documents

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Timeline

PhaseClosed

PostedMay 21

Presolicitation

Response DeadlineJun 10

Deadline has passed

Submission Closed

06/10/2004, 05:00:00 AM UTC

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Organization & Contact Information

AgencyN/A

Contacts1 person available

OfficeN/A

Organization / Agency

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Office AddressN/A

Contacts

Clarice E. DixsonContract Specialist

contact@example.gov

+1 (555) 000-0000

Full Description

The USDA, Agricultural Research Service in Albany, California has a requirement for bi-directional sequencing of polymerase chain reaction (PCR) amplicons [PCR reactions will be performed in-house and no assembly or analysis will be required; these amplicons will be shipped to the vendor for processing]. The sequencing process must be performed in a fully-automated production sequencing facility that tracks the quality and genealogy of all samples in real-time, on a well-by-well basis using a fully-integrated laboratory information management system (LIMS) to ensure accurate genealogy. The quality of pass rates must be 85% - 90% with average PHRED 20 read lengths of 650 - 700 bp. Forward and reverse sequencing reads must be performed within the same sequencing plate to assure a high linkage rate. Detection of sequencing products must be performed on an ABI 3730 so as to share data with on-going sequencing project. Chromatograms, PHRED quality scores and FASTA sequence files must be delivered via FTP. Turnaround time must be within 10 business days for 10,000 or less amplicons.