This Combined Synopsis/Solicitation opportunity from Department Of Agriculture was posted on May 22, 2024. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

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B599 - Genetic Sampling for Tree Species, Rocky Mountain Research Station, Forest Inventory Analysis

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1240BG24Q0027Federal

Submission Closed05/29/2024, 05:00:00 PM UTC

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

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NAICS: 541380

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about 19 hours ago

DEADLINE

in 30 days

General Info

Agency

Department Of Agriculture → Ocp-Pod-Acq-Mgmt-Branch-Ftc USDA

Contract Value

$40,000

NAICS

541380 - Testing Laboratories and Services

Place of Performance

Ogden, UT, USA

Set-Aside

SBA

Awardee

OREGON STATE UNIVERSITY

Award Issued Date

07/10/2024, 12:00:00 AM UTC

Documents

Solicitation 1240BG24Q0027 - Genetic Sequencing for Tree Species

rfq

High

AI Contract Breakdown

Uniform Contract Format

Contract not broken down yet

CLEATUS splits the solicitation into Uniform Contract Format (UCF) sections: scope, pricing, deliverables, and evaluation criteria.

Timeline

3 updates

PhaseClosed

PostedMar 29

special-notice

Notice updatedApr 3

Response deadline changed from May 4, 2024 to Apr 8, 2024. The notice description changed.

Notice updatedApr 30

Notice type changed from Special Notice to Combined Synopsis/Solicitation. Response deadline changed from Apr 8, 2024 to May 22, 2024. The notice title changed. Set-aside added: Total Small Business Set-Aside (FAR 19.5). The notice description changed.

Notice updatedMay 22

Response deadline changed from May 22, 2024 to May 29, 2024. The notice description changed.

Response DeadlineMay 29

Deadline has passed

Submission Closed

05/29/2024, 05:00:00 PM UTC

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Organization & Contact Information

AgencyDepartment Of Agriculture → Ocp-Pod-Acq-Mgmt-Branch-Ftc USDA

Contacts1 person available

OfficeFORT COLLINS, CO, 80521, USA

Organization / AgencyDepartment Of Agriculture → Ocp-Pod-Acq-Mgmt-Branch-Ftc USDA

Office AddressFORT COLLINS, CO, 80521, USA

Contacts

Jason Bologna

contact@example.gov

Full Description

Genetic testing of tree needle samples to determine tree species and provide information regarding their level of certainty in the accuracy for each sample according to the below testing protocol and in accordance with the statement of work included in the attached combined solicitation/synopsis. Testing protocol description: 1. Receive the samples, organize them, prepare for DNA testing and data entry. 2. Extract total genomic DNA from a 1'2 mm segment of a single pine needle from each fascicle using either: i. the Sigma Aldrich Extract?N?Amp Plant PCR Kit (St. Louis, Missouri) or secondarily ii. the Qiagen DNeasy Plant Mini Kit (Valencia, California). 3. Quantify DNA concentration to confirm extraction success. Repeat extraction in case of insufficient DNA. 4. Use the polymerase chain reaction (PCR) to amplify two regions of the pine chloroplast DNA (cp DNA) with diagnostic restriction enzyme cut site differences (i.e., chloroplast matK BsmAl and psbA-trnH Psil loci). 5. Add restriction enzyme to a 10 ?l aliquot of the PCR reaction (restriction digestion). 6. Use agarose gel electrophoresis to visualize the presence/absence of restriction cut sites. a. Presence of a cut site in cpDNA region 1 is diagnostic of whitebark pine. Presence of a cut site in cpDNA region 2 is diagnostic of limber pine. 7. Record cut site results and species ID to whitebark or limber pine in a CSV?formatted file. 8. In case of ambiguities (both or neither region cuts), repeat steps 1?6. If still ambiguous, record as unknown. 9. Record notes relevant to any anomalies found while assessing the species. Examples include, but are not limited to, - - PsiI bands faint ? redo with more DNA in PCR rxn. 3rd BsmAI run again yielded a blank. - - blank on BsmAI 14 July 2021 - - 1 band 6 July 2021 - - needles not fascicled; 3 bands faint 6 July 2021; blank on BsmAI 14 July 2021 - - needles all separate and not in fascicles 10. Return results to RMRS?FIA in the form of a spreadsheet and/or CSV file via email *****05/21/2024 *****(UPDATED) - Amendment 0001 to solicitation #1240BG24Q0027. The purpose of this amendment is to notify the public of the following: 1) This solicitation period is extended until 05/29/2024 at 1:00PM EDT. 2) Questions must be received no later than 05/27/2024 at 1:00PM EDT. 3) Interested vendors are asked to review the testing methods specified in this solicitation at the link included in this notice, reference: (Alongi et.al. 2019)