Benchtop Ultra-Long Read DNA Sequencer

This solicitation, numbered 1232SA26Q0686, is a combined synopsis and request for quotation for a Benchtop Ultra-Long Read DNA Sequencer issued under FAR Part 12 as a commercial item acquisition. The requirement is a Total Small Business Set-Aside under NAICS code 334516 with a small business size standard of 1,000 employees, and only responsible small business concerns may submit quotations. The equipment is needed for de novo genome assembly, genomic analysis, and transcriptomic analysis of native and invasive weed species at the USDA-ARS Wheat Health Genetics and Quality Unit in Pullman, Washington. The sequencer must be new, not refurbished, and capable of producing reads exceeding 20 kilobases with ultra-long reads of at least 2 megabases, supporting direct RNA sequencing without cDNA synthesis, accurate sequencing of GC-rich and repetitive regions, and direct detection of epigenetic modifications such as methylation. It must also generate 5-10x coverage for complex dicot and grass genomes up to 5GB in size, fit within a 300 x 200 x 500 mm footprint, operate on 115 V/60 Hz power, and integrate with existing DNA extraction and library preparation workflows. The device must be installed on-site with a standard one-year manufacturer’s warranty beginning at final acceptance. The contract will be awarded as a Firm Fixed Price on a lowest price technically acceptable basis, with evaluation based on three pass/fail gates: price responsiveness, technical acceptability, and past performance. Quoters must submit pricing for all items and include the place of manufacture for each product. Technical acceptability requires demonstration of compliance through descriptive literature such as datasheets or brochures, and all offers must confirm that the product can be delivered within 60 days after award. F.O.B. Destination is the required delivery point with all shipping costs assumed by the vendor unless F.O.B. Origin is explicitly specified with shipping costs included. The solicitation incorporates multiple clauses including Continuity of Services, Security Requirements, Basic Safeguarding of Information Systems, Preference for Privately Owned U.S.-Flag Vessels, Anti-Discrimination and DEI Compliance, and Disputes Clause provisions. Offerors must certify compliance with Federal anti-discrimination laws, including Title VI and VII of the Civil Rights Act, Executive Orders 14168 and 14173, and cannot