DNA Amplicon Library

This is a combined synopsis/solicitation for commercial service prepared in accordance with the format in FAR subpart 12.06 and 13 Simplified Acquisition Procedures, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. The solicitation number is 12305B21Q0043 and is issued as a request for quotation (RFQ). This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 2021-06. The associated NAICS Code is 541380 and the Small Business Size Standard is $16.5 million. Description of Work: The USDA Agricultural Research Service (ARS) Appalachian Fruit Research Station (AFRS) requires construction of 16s and ITS DNA amplicon libraries for paired end sequencing on an Illumina MiSeq using V2 2x250 sequencing chemistry. Bacterial and fungal amplicon library preparation from 863 genomic DNA samples with standard quality and quantity confirmation and including blocking of contaminant (chloroplast, mitochondria, host) sequences with peptide nucleic acids, library normalization and pooling, confirmation of normalization on the Illumina MinISeq platform using 2x150 sequencing chemistry, paired-end sequencing chemistry on the Illumina MiSeq platform using V2 2x250, complete bioinformatic analysis which includes both bacterial and fungal taxonomic classification of amplicons using the DADA2 pipeline. Genomic DNA samples will be extracted by ARS and shipped to the vendor. Vendor will construct libraries, confirm library quality/quantity, pool libraries, and sequence library pools on an Illumina MiSeq using V2 2x250 sequencing chemistry. Sequence data and will be de-multiplexed and delivered to ARS by providing a link from which the sequence data (in .fastq format) along with output files from the bioinformatic analysis can be downloaded - See Attachment A - Statement of Work Each process required to complete the requirement must be addressed and priced separately in the quote. Period of Performance- Date of award to 09/30/2021 Deliverables data should be within 6 to 8 weeks of submission. Delivery Location: USDA Agricultural Resource Service (ARS), Appalachian Fruit Research Station (AFRS) 2217 Wiltshire Road Kearneysville, WV 2543 EMAIL: contact@example.gov DOCUMENTS TO BE INCLUDED IN QUOTE: In order to be considered for award, Offerors shall respond by: 1) Providing all associated documents identified in the attached clauses and provisions document, specifically 52.212-1 Instruction for the Preparation of Offers (Addenda). 2) Have an Active SAM.gov registration and complete the Representations in FAR 52.204-54; the Representation and Certification section should also be completed electronically in the System for Award Management (SAM) at www.sam.gov. Any amendment and documents related to this procurement will be available electronically at the Government Point of Entry (beta.sam.gov). FAR clauses 52.212-1, 52.212-2, 52.212-3, 52.212-4 and 52.212-5 (and any addenda to those clauses) are included in the attached clauses and provisions document. Additionally, other contract requirements and terms/conditions can be located in the same document. Electronic responses are mandatory. Information provided will not be used as a part of any subsequent solicitation requirement. Interested vendors who submit data are responsible for appropriately marking information if it is proprietary in nature. A determination by the Government not to award this proposed contract action, based on this notice, is solely within the discretion of the Government.