H9-Specific ddPCR Primer and Probe Design

The contract involves designing and validating primers and probe sets that are specific to the H9 human neural stem cell line for use in droplet digital PCR (ddPCR) assays. These primers and probes must demonstrate high specificity to the target human cell line and avoid cross-reactivity with African green monkey genomic DNA. The project is a subcontract opportunity issued by the Department of Veterans Affairs through the 262-NETWORK Contract Office 22, with the work to be performed in San Diego, California. This opportunity is designated as a Service-Disabled Veteran-Owned Small Business (SDVOSB) set-aside under NAICS code 541620. The solicitation was posted on April 16, 2026, with responses due by May 18, 2026. The effort focuses on advancing molecular assay tools specific for neural stem cell research, reflecting the government’s interest in precise and reliable diagnostic reagents in healthcare-related biotechnology applications.