Harmful Algal Bloom (HAB) ddPCR and RT-ddPCR Laboratory Analysis

The contract requires the execution of digital droplet PCR and reverse transcription digital droplet PCR analyses on samples collected from harmful algal blooms to detect the presence of the anaC gene, a marker associated with harmful toxin-producing organisms. The work encompasses the full workflow including extraction of DNA and RNA from environmental samples, molecular amplification and quantification using ddPCR and RT-ddPCR platforms, and stringent quality assurance measures to ensure accuracy, reproducibility, and compliance with analytical standards. All procedures must be performed under controlled laboratory conditions with documented protocols to maintain data integrity and meet regulatory expectations for environmental monitoring. This is a small business set-aside subcontract under NAICS code 541380, allocated by the Office of Acquisition Grants within the Department of the Interior, with a response deadline of June 30, 2026. The work is performance-based and will involve compliance with established laboratory protocols for molecular diagnostics in environmental samples. The successful offeror is expected to deliver reliable, high-quality analytical results that support monitoring and management efforts for harmful algal blooms, with emphasis on precision, contamination control, and traceability of results throughout the testing process.