Long-read genome sequencing for the identification of genetic markers for Highly Pathogenic Avian Influenza resistance enabling crossbred genomic evaluation and feed efficiency in dairy and beef cattle

This contract involves long-read genome sequencing projects managed by the Department of Agriculture's Agricultural Research Service to advance livestock genomic research. The primary objective is to utilize ultra-long DNA reads to assemble complex regions of livestock genomes, which will facilitate the identification of genetic markers for resistance to Highly Pathogenic Avian Influenza. Additionally, the sequencing efforts aim to support the construction of a dairy pangenome for crossbred genomic evaluations and enhance the scientific understanding of feed efficiency in both dairy and beef cattle. The technical scope of the work requires performing long-read DNA sequencing using Oxford Nanopore and Pacific Biosciences technologies on 82 cattle and various tissue samples. The resulting data will be used to generate comprehensive genome assemblies for all 82 animals, providing the foundational information necessary to support four distinct research projects. This forecast, categorized under NAICS code 541380, serves as a precursor to future procurement activities related to these genomic advancements.