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This Government Contract opportunity was posted on May 9, 2017. The submission period has ended. Browse the details below for market research, or find similar active opportunities.

Metratranspirtomics analysis of 32 RNA samples

Awarded
850965Federal

Contract Overview

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The U.S. Department of Agriculture Agriculture Research Service is seeking a small business vendor under NAICS code 541711 to perform metratranscriptomics analysis on 32 existing intestinal RNA samples. The scope of work includes quality control testing, rRNA removal, cDNA library preparation, and sequencing using an Illumina HiSeq platform with a PE150 bp strategy. Additionally, the vendor must provide comprehensive bioinformatics and statistical analysis focusing on gene expression within the gut microbiome and host, concluding with a summary of highlighted mRNA differences. All services must be completed within 50 business days following the submission of the library preparation confirmation. Samples must meet specific quality thresholds, including a minimum volume of 2.5 ul, a concentration of at least 65 ng/ul, and a purity level of OD260/280 of 1.8 or higher without degradation or DNA contamination. Eligible small business vendors were required to submit quotes and an active 9-digit Dun and Bradstreet number by May 1, 2017, for an intended award date of May 9, 2017.

General Info

Agency

N/A

Contract Value

$28,150

NAICS

541711 - Research and Development in Biotechnology

Place of Performance

USDA, ARS, NEA, ABBL 10300 Baltimore Ave. Building 1043, Rm. 6, Barc-East, BELTSVILLE, MD, 20705, USA

Set-Aside

NONE

Award Issued Date

Documents

0

No documents available

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Timeline

PhaseAwarded
Posted

Combined Synopsis

Awarded

Contract was awarded

Submission Closed

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Organization & Contact Information

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AgencyN/A
Contacts2 people available
OfficeN/A
Organization / Agency
N/A
Office AddressN/A
Contacts
John C. WilkinsonPurchasing Agent
Lori A. FaberSupervisory Contract Specialist

Full Description

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Referencing Solicitation Number 850965, This is a Combined Synopsis/Solicitation for the following requirement for the U.S. Department of Agriculture ( USDA ), Agriculture Research Service ( ARS ), who intends to award a fixed price purchase order to the small business vendor that can meet the following need: The applicable North American Industrial Classification Code (NAICS Code) is: 541711 with a small business size standard of 1,000 employees. This requirement is reserved exclusively for small businesses as listed above. USDA's Requirement is as follows: USDA is requiring 32 existing intesitinal RNA samples for metratranscriptomics analysis. Each of the 32 above listed samples shall require quality control testing, remove rRNA from each sample. Shall make cDNA library and sequencing, and prepare a bioinformatics analysis with gene expression in the gut microbiome and host, and shall provide statistical analysis for each group and shall also provide a summary of highlighted mRNA differences. This requirement shall provide transcriptome sequencing and RNA-Seq quantification using an Illumina, Inc. manufactured HiSeq platform with PE150 bp sequencing strategy. All Services must be provided 50 business days after USDA receives the library preparation confirmation form the vendor. Sample requirements are as follows: Greater than or equal (Amount) (Quibit) 2.5 ul., with a concentration of greater than or equal to 65 ng/ul.; and a purity level of OD260/280 of greater than or equal to1.8 with no degradiation or DNA Contamination. All responsible ( small business) vendors submitting a quote on the requirement, must include their companies 9 digit Dun and Bradstreet number; which must be ( both) current and active at the intended award date of May 9, 2017. Please submit Your quotes and Dun and Bradstreet number via email to: John.Wilkinson@ars.usda.gov by No later than May 1, 2017

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