Protein Interactors Using a Yeast-Based System

This is a Full & Open Notice for brand name or equal Hybrigenics ULTimate Y2H and Y2H+1 in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals that is being requested and a written solicitation will not be issued. Solicitation No. AG-5615-S-17-BB01 is issued as a Request for Proposals (RFP). This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-26. The NAICS code applicable to this acquisition is 541711. The USDA, ARS, MWA, Vegetable Crops Research Unit, Madison, WI has a need for a screen for protein interactors using a yeast-based system with the ability to search for direct interacting proteins (yeast 2-hybrid) as well as the ability to search for interacting proteins when a third protein of interest is present (yeast 3-hybrid). The requirement is for at least 20 genes from Nicotian benthamiana that encode proteins and interacts with the Phytophthora infestans effector protein IPI-O1 in the absence of the Solanum tuberosum protein RB, and at least 20 genes from Nicotiana benthamiana that encode proteins that interact with IPI-O1 in the presence of RB. This system will be used to identify plant proteins that interact with the Phytophthora infestans effector protein IPI-O1. This effector is required for infection of potato and other host plants. The corresponding resistance protein from potato, called RB, recognizes the presence of the IPI-O1 effector to elicit resistance. We require additional information about the function of IPI-O1 in the infection process, including what host proteins it interacts with. This will give us an idea of what its targets are and, therefore, an idea of how it is used to cause disease. THE SALIENT CHARACTERISTICS: The Nicotiana benthamiana system must meet these minimum requirements: 1) Have access to an existing cDNA library from Nicotiana benthamiana, 2) Be able to complete a screen to identify direct interactions between a single bait protein and a library of putative interacting proteins, 3) Be able to complete a screen to identify direct interactions between a single bait protein and a library of putative interacting proteins in the presence of a third protein, 4) Use a yeast mating protocol to screen a large number of individual clones, 5) Be able to screen at least 10 million independent gene fragments, 6) Able to test all protein domains for interaction with the bait protein, 7) Sequence and analyze all clones identified in the screen, 8) Provide a comprehensive bioinformatics analysis of identified interacting proteins, 9) Provide a confidence score with each interacting protein. DOCUMENTS TO BE INCLUDED IN PROPOSAL: In order to be considered for award Offerors shall provide 1) A quote on company letterhead detailing the item description, unit price and total price; 2) Descriptive literature, brochures, information on service and support; 3) At least 3 references regarding this type of research. References must have proposed research within the last three (3) calendar years. References shall be provided for the product proposed and if possible include the email address of the person to be contacted; and 4) Offerors shall include a completed copy of the provisions FAR 52.212-3, OFFEROR REPRESENTATIONS AND CERTIFICATIONS - COMMERCIAL ITEMS, with this offer; a copy of this provision may be obtained at https://acquisition.gov/comp/far/loadmainre.html. REJECTION OF PROPOSAL: Failure to demonstrate compliance will be cause to reject the quote without further discussions. All responsible sources may submit an offer which will be considered. The following provisions and clauses apply to this acquisition and can be found in full text at https://acquisition.gov/comp/far/index.html. FAR 52.252-1, Solicitation Provisions Incorporated by Reference are as follows: FAR 52.204-7, FAR 52.204-10 System Award Management database (ALL OFFERORS MUST BE REGISTERED IN THE SYSTEM AWARD MANAGEMENT DATABASE IN ORDER TO RECEIVE AN AWARD). The website address for registration is: https://www.sam.gov; and FAR 52.222-22, Previous Compliance Report, FAR 52.252-2, Clauses Incorporated by Reference are as follows: FAR 52.212-4, Contract Terms and Conditions Commercial Items; FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items apply to this acquisition. The following FAR clauses cited in 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items are applicable to this acquisition: FAR 52.222-3, Convict Labor (E.O. 11755): FAR 52.222-19, Child Labor; 52.222-21, Prohibition of Segregated Facilities; 52.222-26, Equal Opportunity; 52.222-35, Equal Opportunity for Special Disabled Veterans; 52.222-36, Affirmative Action for Workers with Disabilities; 52.222-37, Employment Reports on Special Disabled Veterans; 52.225-13, Restriction on Certain Foreign Purchases; 52.232-33, Payment by Electronic Funds Transfer-System Award Management (31 U.S.C. 3332); 52.232-35, Payment by Third Party (31 U.S.C. 3332) and FAR 52.233, Protest After Award. The above FAR clauses may be viewed at http://www.acquisition.gov/comp/far/loadmainre.html. SHIPPING: FOB Destination. DELIVERY TO: USDA-ARS, Vegetable Crops Research Unit, Madison, WI 53706. PROPOSAL PRICE MUST INCLUDE: shipping or handling fees, as well as any applicable duties, brokerage, or customs fees. DELIVERY DUE DATE: The Government requires delivery within three (3) months after award and PO is issued, however, each offeror shall include their proposed delivery schedule as part of their quote. FAR 52.212-2, EVALUATION - COMMERCIAL ITEM, the significant evaluation factors, in the relative order of importance, are (i) technical capability of the item offered to meet the Government requirement including delivery date; (ii) past performance; and (iii) price (based on F.O.B. Destination), technical and past performance are more important than price. Award will be made based on overall BEST VALUE to the Government. EVALUATION & AWARD FACTORS: The contract resulting from this solicitation will be awarded to the responsible party whose offer, conforming to the solicitation, is determined most advantageous to the government, cost, and other factors considered. Each Proposal shall respond to each of the evaluation factors identified below. With the exception of past performance, a technical evaluation will be performed on each quote based solely on the information furnished and not on previous knowledge or associations. The evaluation factors are as follows: (1) Technical Evaluation Factors: A) All quotes will be rated against the salient characteristics and minimum requirements, B) Delivery, Any amendment and documents related to this procurement will be available electronically at the Internet site: http://www.fbo.gov. Potential offerors will be responsible for downloading their own copy of those documents related to this procurement, if any. Furnish Proposals to Dolores Milton, Contracting Officer, USDA- ARS, Administration Office, Dairy Forage Research Center, 1925 Linden Drive, Madison, WI 53706-1108, no later than 4:00 p.m. April 28, 2017. Proposals and other requested documents may be provided by facsimile to +1 (555) 000-0000 or if desired Email proposals to: contact@example.gov Subject: Plant Proteins FBO. Additional information may be obtained by contacting the Contracting Officer at +1 (555) 000-0000 or Email.