Recombinant Protein Expression and Purification of 3XFLAG-tagged sdAbs

The contract involves the expression and purification of 0.5 to 1 milligram each of four validated single-domain antibody clones tagged with a 3XFLAG sequence. These antibodies are to be produced using either prokaryotic or eukaryotic expression systems, followed by affinity purification and buffer exchange to ensure high-quality protein preparation. The work is designated as a subcontract and is set aside exclusively for small business participation under the SBA Total Small Business Set-Aside program. This work is solicited by the National Institutes of Health, part of the Department of Health and Human Services, with the performance location in Bethesda, Maryland (zip code 20817). The contract calls for efficient production of recombinant proteins critical for research or therapeutic applications, emphasizing the use of validated clones and adherence to expression and purification standards to deliver purified proteins in the specified quantities. The response deadline for this opportunity is May 24, 2026.