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Replacement of Non-functional Nikon TIRF Microscope – LCMB Microscopy Core Facility, NIH/NCI

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75N98026Q00774Federal

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The contract is for the replacement of a non-functional Nikon TIRF microscope system at the LCMB Microscopy Core Facility, NIH/NCI, under solicitation number 75N98026Q00774. The new system includes a comprehensive bundle of advanced components: a Ti2E Motorized PFS Microscope with an encoded stage and fluorescence filter sets for DAPI, GFP, and DS Red; a Nikon Motorized E-TIRF Package with a barrier filter wheel; an ORCA-FusionBT sCMOS camera with USB3.0 and dual camera interface; an NI-BB Plus analog triggered device hub; a LUNF STORM multi-line laser unit operating at 405/488/561/640 nm; a Ti2 motorized main and sub-branch with a replaceable 100% reflective mirror; a C-NSTORM Quad filter set; a Ti2-NS2-MU N-STORM magnification change unit; an NIS-Elements software suite with six Advanced Research licenses and HASP reconfiguration; and a Z4 Advanced Imaging Workstation V8 equipped with a 31.5-inch 4K Thunderbolt 3 display. The installation kit is included, along with the reintegration of existing functional components such as the CFI60 Apochromat TIRF 100X Oil Immersion Objective, MCL 100-micrometer piezo nanopositioner, EMCCD camera, C-NSTORM 647 filter set, C-FL TIRF CFP/YFP filter set, and NIS-Elements STORM software licenses, all at no additional cost to the client. All system components must be delivered within 90 to 120 days after contract award to the specified address in Building 37, Room 2033B1 at the NIH campus in Bethesda, Maryland. Upon delivery, the contractor is required to immediately coordinate with the NCI Project Officer to schedule installation, which includes full optical alignment, integration of all new and existing components, and verification of full system performance to manufacturer specifications. The system must be fully operational and ready for use within 14 days of delivery. Comprehensive onsite training for the LCMB Microscopy Core personnel is mandatory following installation to ensure proficient operation of the complete system. The contract is issued by the National Institutes of Health under the Department of Health and Human Services, with John

General Info

Replace Nikon TIRF microscope with advanced components, deliver in 90–120 days, install and train staff at NIH Bethesda.

Agency

Department Of Health And Human Services → National Institutes Of Health OlaoView Agency

NAICS

334516 - Analytical Laboratory Instrument ManufacturingView NAICS

Place of Performance

MD, 20892, USA

Set-Aside

NONE

Documents

(1)

Requirement rev 12 - Ti2E Motorized PFS Microscope Bundle

DOCXrequirement

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Organization & Contact Information

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AgencyDepartment Of Health And Human Services → National Institutes Of Health Olao
Contacts1 person available
OfficeBETHESDA, MD, 20892, USA
Organization / Agency
Department Of Health And Human Services → National Institutes Of Health Olao
View Agency Profile
Office AddressBETHESDA, MD, 20892, USA
Contacts

Full Description

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  1. PRODUCT FEATURES/SALIENT CHARACTERISTICS 


The following product features and characteristics are required for this requirement: 


  • Ti2E Motorized PFS Microscope Bundle with motorized encoded stage and fluorescence filter sets (DAPI, GFP, DS Red) 


  • Nikon Motorized E-TIRF Package with Ti2-P-FWB-E barrier filter wheel 


  • ORCA-FusionBT sCMOS camera with USB3.0 interface card and dual camera interface kit 


  • NI-BB Plus analog triggered device hub 


  • LUNF STORM 405/488/561/640 multi-line laser unit 


  • Ti2 motorized main branch and sub branch with exchangeable 100% reflective mirror 


  • C-NSTORM Quad 405/488/561/640 filter set 


  • Ti2-NS2-MU N-STORM magnification change unit 


  • NIS-Elements software package with Advanced Research licenses (×6) and HASP reconfiguration 


  • Z4 Advanced Imaging Workstation V8 with Thunderbolt 31.5-inch 4K display 


  • Installation kit 


  • Reintegration of the following existing components from the current instrument at no additional cost to CCR/NCI: CFI60 Apochromat TIRF 100X Oil Immersion Objective Lens (N.A. 1.49), MCL 100 micrometer piezo nanopositioner with controller and electronics, EMCCD camera, C-NSTORM 647 filter set, C-FL TIRF CFP/YFP filter set, and NIS-Elements STORM acquisition and analysis software licenses 


  1. DELIVERY / INSTALLATION 


Contractor shall deliver all system components within 90–120 days after award to: 


Sandeep Pallikkuth,  


LCMB, CCR, NIH/NCI  


37 Convent Dr. MSC 4256  


Building 37, Room 2033B1  


Bethesda, Maryland 20892 


Upon award and delivery, the contractor must notify the NCI Project Officer, Sandeep Pallikkuth, to schedule an installation date and time. Installation must include full optical alignment, integration of all new components, reintegration of reused components from the existing instrument, and verification of complete system performance to manufacturer specifications. The Contractor shall install the system and have it ready for use within fourteen (14) days after delivery. 


4.3 TRAINING 


Onsite training of LCMB Microscopy Core personnel on operation of the complete system is required following installation. 

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