DNA Extraction and Shotgun Metagenomics Sequencing of Fecal Samples

The National Institutes of Health intends to award a sole-source contract to Rush University Medical Center for DNA extraction and shotgun metagenomics sequencing of 200 fecal samples—100 from humans undergoing a ketogenic diet with and without NR supplementation and 100 from mice on a high-fat diet with and without NR supplementation. This acquisition is justified under FAR 6.302-1 due to the unique capabilities of Rush University Medical Center’s Genomics and Microbiome Core Facility, which are not available from any other source, making full and open competition impractical. The work will support cross-species validation of mechanisms involving bile acid signaling and FXR activation in promoting ketogenesis. Deliverables include complete sample processing from DNA isolation through library preparation and sequencing on a NovaSeq X platform, with all raw sequencing data—approximately 930 GB—delivered to Dr. Katherine Maki at NIH in Bethesda, Maryland. The estimated cost for the services is $24,242, broken down into line items covering DNA extraction, library preparation, and sequencing, though this amount is provisional and reflects a proposed vendor quote rather than a formally obligated contract value. This is a Notice of Intent, not a solicitation, and no formal competitive process is being conducted. Responsible sources wishing to be considered must submit a capability statement via email to valerie.gregorio@nih.gov by 12:00 PM Eastern Time on May 23, 2026, using the subject line NOI-CC-OLAO-26-00551. While no evaluation factors, contract clauses, packaging standards, inspection criteria, or formal certifications are specified, the government retains sole discretion to determine whether competition is warranted based on responses. The place of performance is Bethesda, Maryland, and payment details are directed to Rush University Medical Center via ACH to a designated account. There is no defined period of performance, FOB terms, or contract type listed, and the acquisition follows the statutory authority of 41 U.S.C. 253(c)(1). No formal submission format, page limits, or file type requirements are enforced for the capability statement, and no additional special requirements such as OCI controls, security clearances, or option periods are included.