Innate Immunity Analysis mRNA Sequencing Services

FedBizOps Synopsis Document Type: Combined Synopsis/Solicitation Solicitation Number: RFQ-NIAID-2048638-R1 Posted Date: November 10, 2020 Response Date: November 15, 2020 Set Aside: None NAICS Code: 541380 Classification Code: Q301 Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Institute of Allergy & Infectious Diseases/AMOB, 903 S 4th Street, Hamilton, MT 59840 Title: Innate Immunity Analysis mRNA Sequencing Services Primary Point of Contact: Dana Monroe; contact@example.gov Description: This notice is a combined synopsis/solicitation for commercial items using Simplified Acquisition Procedures. This acquisition will be made in accordance with the format in Federal Acquisition Regulation (FAR) Subpart 12.6 - Streamlined Procedures for Evaluation and solicitation for commercial Items, as applicable, and as supplemented with additional information included in this notice. This announcement constitutes the only solicitation (a written solicitation will not be issued) and quotes are being requested. The Solicitation number is RFQ-NIAID-2048638.R1 and the solicitation is issued as a Request for Quotes (RFQ). This acquisition will be processed under FAR Part 13 Simplified Acquisition Procedures (SAP). The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2021-01 October 01, 2020. The North American Industry Classification System (NAICS) code for this procurement is 541380, Testing Laboratories, with a small business size standard of $16.5 million. The requirement is being competed full and open competition, un-restricted and without a small business set-aside. The National Institute of Allergy and Infectious Diseases (NIAID) has a requirement for: This requirement is to send 72 samples of RNA from cells that are MTB-negative or Mtb-infected from non-human primate granulomas for RNA-sequencing to allow for evaluation of innate immune pathways specific to pathways that change upon infection with Mtb. This requirement is to send 72 samples of RNA from cells that are MTB-negative or Mtb-infected from non-human primate granulomas for RNA-sequencing to allow for evaluation of innate immune pathways specific to pathways that change upon infection with Mtb. We require Ultra-low RNA sequencing We require the NovaSeq PE150 read size or larger (larger fragments of DNA map to the genome with fewer false positive errors than smaller fragments) We require a turnaround time of 45 working days after receipt of sample QC report from NIH We require a company that is willing to accept samples that were infected with a BSL3 pathogen, Mtb, but have been decontaminated We require enough depth and breathe of reads for us to - see all the genetic changes. Most companies are selling 6G of raw data, but we require 9G of raw data per sample that will give us the coverage we need We require a company that can sequence all 72 samples at the same time- running samples in batches increases - noise in the sequencing that may result in false readings of gene changes We require quantification analysis of the data, which requires bioinformatics knowledge and computers with sufficient processing power to run these analyses Samples that are outside of QC criteria or expectation and the investigators decided not to pursue will not be charged against the PO We require a company that can sequence samples from 11-500 NHP (Rhesus Macaque) cells where the total RNA <1ng Place of Performance: NIH, 33 North Drive, Bldg 33, Bethesda, MD 20852, United States. FOB: Destination The government intends to award a firm fixed price purchase order to the responsible contractor as a result of this RFQ that will include the terms and conditions set forth herein. The award will be based on the following; technical capability to meet the requirements and price. The following FAR provisions apply to this acquisition: FAR 52.212-1 Instructions to Offerors Commercial Items (June 2020) FAR 52.212-3 Offerors Representations and Certifications - Commercial Items (AUG 2020) FAR 52.204-7 System for Award Management (Oct 2018) (Offerors must complete annual representations and certifications on-line at http://www.sam.gov/ in accordance with FAR 52.212-3 Offerors Representations and Certifications- Commercial Items (Please ensure 52.209-2 -- Prohibition on Contracting with Inverted Domestic Corporations--Representation. (Nov 2015) is updated in your SAM record) FAR 52.203-18 Prohibition on Contracting with Entities that Require Certain Internal Confidentiality Agreements - Representation (Jan 2017) 52.204'24 Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment (AUG 2020) *** Must complete representation if attached to this solicitation- see attachment 52.204-24 The following FAR contract clauses apply to this acquisition: FAR 52-212-4 Contract Terms and Conditions Commercial Items (OCT 2018) FAR 52-212.5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders Commercial Items (OCT 2020) *The applicable subparagraphs of FAR52.212-5 are included in the attachment to this posting. FAR 52.232-40 Providing Accelerated Payments to Small Business Subcontractors (Dec 2013) FAR 52.204-13 System for Award Management Maintenance (Oct 2018) HHSAR 352.222-70, Contractor Cooperation in Equal Employment Opportunity Investigations (December 2015) 52.252-2 -- Clauses Incorporated by Reference. (Feb 1998) This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at this address: http://farsite.hill.af.mil/vffara.htm By submission of an offer, the offeror acknowledges the requirement that a prospective awardee shall be registered in the System for Award Management (SAM) database prior to award, during performance, and through final payment of any contract, basic agreement, basic ordering agreement, or blanket purchasing agreement resulting from this solicitation (www.sam.gov). Copies of the above-referenced provisions and clauses are available from http://www.acquisition.gov/far/loadmainre.html & http: //www.hhs.gov/regulation/index.html or, upon request, either by telephone or fax. Submission shall be received not later than November 15th, 2020 at 5:00pm EST Offers may be e-mailed to Dana Monroe (email: contact@example.gov). Offers shall include RFQ number in the subject line (RFQ-NIAID-2048638-R1). Late submissions shall be treated in accordance with the solicitation provision at FAR 52.212-1(f) All responsible sources may submit an offer that will be considered by this Agency. Any questions or concerns regarding this solicitation should be forwarded in writing via e-mail to Dana Monroe at contact@example.gov.