Metabolic Services

Description SOURCES SOUGHT NOTICE IAS REQ. 1041303 THIS IS A SOURCES SOUGHT ANNOUNCEMENT ONLY. This notice does not constitute a commitment by the Government. All information submitted in response to this announcement is voluntary, and the Government will not pay for information requested nor will it compensate any respondent for any cost incurred in developing information provided to the Government. The United States Department of Agriculture (USDA), Agricultural Research Service (ARS) is searching for firms who can provide a service contract labeled as follows: Statement of Work - Title: Metabolic Services Colorado State - Metabolomics Analysis As in past analyses, the Facility will perform the following: Liquid Chromatography Mass Spectroscopy. Inject 810 aqueous samples into a Waters Acquity UPLC CSH Phenyl Hexyl column (1.7 ?M, 1.0 x 100 mm) using a gradient from solvent A (2mM ammonium hydroxide, 0.1% formic acid) to solvent B (Acetonitrile, 0.1% formic acid) for separation. Gas Chromatography Mass Spec. 40 ?L aliquot of the aqueous phase re-suspension was dried under nitrogen, re-suspended in 50 ?L of pyridine containing 25 mg/mL of methoxyamine hydrochloride. Detect metabolites using a Trace 1310 GC coupled to a Thermo ISQ mass spectrometer (Thermo Scientific). Separate using a 30 m TG-5MS column (Thermo Scientific, 0.25 mm i.d., 0.25 ?m film thickness) with a 1.2 mL/min helium gas flow rate. Analyses and annotations. Define molecular features by retention time and mass-charge. Use the matchedFilter algorithm for GC-MS data, and the centWave algorithm for LC-MS data. Use RAMClustR to group features. Annotate GC-MS features by searching the GOLM metabolome database using the RAMSearch program. Annotate LC-MS features using RAMSearch and searches against the MassBank database using MSFinder. Responses to this Sources Sought are not quotes on which USDA ARS can issue any contract. This Sources Sought is issued for information and planning purposes only and does not itself constitute a RFQ. The Government does not intend to award a contract or BPAs based only on responses to this Sources Sought. All information received in response to this Sources Sought marked "Proprietary" will be protected and handled accordingly. Interested parties are responsible for adequately marking proprietary or competition sensitive information contained in their response. Responses to the Sources Sought will not be returned. Responders are solely responsible for all expenses associated with responding to this Sources Sought. USDA ARS will not pay for information received in response to this Sources Sought. Do not submit pricing information in response to this Sources Sought. Please send responses with the subject heading - METABOLIC SERVICES to John Wilkinson at John.Wilkinson@usda.gov by 12:00pm by noon EST on July 26, 2021. Please include your companies 9 digit Dun and Bradstreet No. which must (mandatorily ) be registered as current and active by July 26, 2021 by 4:00pm est at the web beta.sam.gov