This Government Contract opportunity from Department Of Defense was posted on July 27, 2026. The submission period has ended. Browse the details below for market research, or find similar active opportunities.
Primers and probes
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NAMRU SOUTH is seeking approval to procure a specialized set of molecular primers and probes for use in the Global Travelers Diarrhea Study, a multisite research effort designed to standardize laboratory methods and assess the epidemiology and etiology of acquired diarrhea among U.S. military and civilian travelers. The contract covers the acquisition of 48 distinct primer and probe sequences targeting specific bacterial genes associated with enteric pathogens, including virulence factors such as aggR, eae, bfpA, Stx1, Stx2, and various housekeeping or selection markers like lacY, uidA, rfc, wbgZ, rfpB, gyrA, and others. Each sequence is specified with exact nucleotide sequences, fluorescent dye labels such as FAM, ATTO550N, HEX, Cy5, CAL Fluor Red 610, CAL Fluor Gold 540, and TexasRed, along with quenchers like MGBNFQ, BHQ_1, BHQ_2, and IAbRQSp to ensure precise detection in multiplex PCR assays. Quantities vary per target, with most sequences ordered in units of two, while key markers like bfpA, GFP, and several DLO probes are ordered in quantities of four to support higher throughput or replicate testing. The procurement is solicited under BAC260012 with a response deadline of August 10, 2026, and is designated as an 8(a) Sole Source contract under NAICS code 423450, indicating it is reserved for small disadvantaged businesses. The contracting office is located at the Naval Medical Research Center in Silver Spring, Maryland, with performance occurring in Bellavista, Callao, Peru. Primary point of contact is Luciana Encinas, reachable by email and phone, with Nataki Johnson as secondary liaison. The contract supports critical global health research under the Department of Defense, enabling precise pathogen detection in traveler-diarrhea cases to inform public health strategies and improve diagnostic standardization across international study sites.
General Info
Agency
NAICS
Place of Performance
BELLAVISTA, PE-CAL, CALLAO 02, PERSet-Aside
Timeline
Submission Closed
Organization & Contact Information
Full Description
NAMRU SOUTH requests approval to award the purchase of primers and probes for protocol “The Global Travelers Diarrhea (GTD) Study: an evaluation of study design and laboratory methodologies standardization in a multisite protocol assessing the epidemiology and etiology of acquired diarrhea among a U.S. military or civilian travelers.
3. Description of Supplies or Services:
2 units HLT-F– TTC CCA CCG GAT CAC CAA
2 units HLT-R– CAA CCT TGT GGT GCA TGA TGA
2 units LT-pro – 5 ATTO550N/CTT GGA GAG AAG AAC CCT /MGBNFQ
2 units STh -F– GCTAAACCAGYAGRGTCTTCAAAA
2 units STh -R– CCCGGTACARGCAGGATTACAACA
2 units STh -pro – 5SUN-TGGTCCTGAAAGCATGAA-3MGBMGBNFQ
2 units STp -F – TGAATCACTTGACTCTTCAAAA
2 units STp -R – GGCAGGATTACAACAAAGTT
2 units STp -pro – 56FAM-TGAACAACACATTTTACTGCT-3MGB-NFQ
2 units virB-F – GGA TTT GTG CAA CGA CTT GTT AAG
2 units virB-R– GAG GAA TCT TGG CTT TGA TAA AGG
2 units virB-pro – /56FAM/ CAC TCC ATT CTG GTA ATA A /MGBNFQ/
2 units aggR-333F– CAG CGA TAC ATT AAG ACG CCT AAA G
2 units aggR-448 R– CGT CAG CAT CAG CTA CAA TTA TTC C
2 units aggR-pro – /FAM/ CTT GCA GTT GTC CGA ATT /MGBNFQ/
2 units eae-F– CCG ATT CCT CTG GTG ACG A
2 units eae-R– CCA CGG TTT ATC AAA CTG ATA ACG
2 units eae-pro –5 ATTO550N/iCY3/ CGT CAT GGT ACG GGT AA /MGBNFQ/
4 units bfpA-Fw -GGT CTG TCT TTG ATT GAA TCT GCA
4 units bfpA-Rv-GCA GAC TGG TAG TAA AAC ATC ACA CC
4 units bfpA-pro-/TexasRed/ GCG CTT GCT GCC ACC GTT ACC G /3BHQ_2/
2 units Stx1-414 F– ACT TCT CGA CTG CAA AGA CGT ATG
2 units Stx1-545 R – ACA AAT TAT CCC CTG AGC CAC TAT C
2 units Stx1-pro – /56FAM/ CTC TGC AAT AGG TAC TCC A /MGBNFQ/
2 units HStx2-F– CCA CAT CGG TGT CTG TTA TTA ACC
2 units HStx2-R – GGT CAA AAC GCG CCT GAT AG
2 units Stx2-pro - /5SUN/ TTG CTG TGG ATA TAC GAG G /3MGBNFQ/
4 units GFP-F-AGA TGA CGG GAA CTA CAA G
4 units GFP-Rv-CCT TCA GCT CGA TTC TAT T
4 units GFP-pro-/5Cy5/CA CCT TCG AAC TTG ACT TCA GCG C/3IAbRQSp/
4 units Blocks Gene fragment-TTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATCTTTCAAAGATGACGGGAaCTACAAGACA CGcGCTGAAGTCAAGTTcGAAGGTGATACCCTTGTTAATAGAATCGAGcTgAAgGGTATTGATTTTAAAGAAGATGGA AACATTCTTGGACACAAATTGGAATACAACTTTAACTCACACAATGTATACATCACGGCAGACA
2 units lacY-F- ACCAGACCCAGCACCAGATAAG
2 units lacY-R -CTGCTTCTTTAAGCAACTGGCGA
2 units lacY-DLO-RFB-5 (3)-pro-FAM/CA TAC ATA TTG CCC GCC AGT ACA GAC /3BHQ_1 (3)
2 units uidA-F-GTGTGATATCTACCCGCTTCGC
2 units uidA-R - AGAACGGTTTGTGGTTAATCAGGA
2 units uidA-DLO-HB1-5-pro-HEX/TC GGC ATC CGG TCA GTG GCA GT/3BHQ_1
2 units rfc-F – ACTTATCATTGACTAGCGAGTTCT
2 units rfc-R – CGCAAGCACTATTATGAGACATTT
2 units rfc-DLO-RFB-5-pro-FAM/TG GTG TCG GTG CGA TTA TCA GTT T/3BHQ1/
2 units wbgZ-F – CAGCGTCAGAATCGCTTACA
2 units wbgZ-R – TCAACCAGAGGCACATGTTTAT
2 units wbgZ-DLO-CAB2-5-pro-CAL Fluor Red 610/TT CAA ACG GTT TAT CAT GCG GCC G/3BHQ2/
2 units rfpB-F – ACCAAGAAAGGGATTTCAGTCTAT
2 units rfpB- R – AAGGTTAATACCACTGCGACTT
2 units rfpB-DLO-CGB1-5-CAL Fluor™ Gold 540/AG CAG GCG CCA TTC CTG TTG ATT A/BHQ1
2 units SBO-3648-F– TTGAGCACGGAAACAGAGAG
2 units SBO-3648-R – TTCCCGTTCTTCCCTTTACAC
2 units SBO-3648-DLO-C3B2-5-pro-Cy5/TG CCG CTT CAC ATA AAT CAA CGC C/3BHQ2/
2 units gyrA-F-CTATAACAACTGCACCTACTAAT
2 units gyrA-R-ATGAAATTTTTGCCAGTGGTG
2 units gyrA-pro-FAM/CT TAA TAG CCG TCA CCC CAC /3BHQ_1
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