Sequencing Genomes of Cattle and Swine

The USDA, Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC) has a project that requires sequencing genomes of cattle and swine. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR subpart 12.6 (current as of FAC 2001-24), as supplemented with additional information included in this notice. This announcement constitutes the only solicitation quotations are being requested and a written solicitation will not be issued. The NAICS is 541380. The Solicitation number AG6538S140010 is issued as a Request for Quotation (RFQ). The description of items and clauses incorporated in this synopsis/solicitation are applicable to this procurement and will be incorporated into any resultant purchase order. Contract Line Item Numbers (CLINs): CLIN 001 - This is a source of supply contract. Multiple orders may be placed from this contract based upon the pricing provided. The maximum value of orders made under this contract will be $149,000. The minimum value of this contract will be $25,000. The project requires sequencing genomic DNA from cattle or swine. Libraries shall be prepared for sequencing on the illumina HiSeq platform, and may be pooled as needed on the instrument. The pools should be sequenced with read lengths of 200 bases or more (2x100 paired end reads are acceptable). Sequence output should be a minimum of 0.5 Terabase of total sequence with acceptable (>Q30) quality per instrument run (2 flow cell, 16 channel high yield run), with no specific requirement for yield per library (i.e. failed libraries do not have to be rerun as long as they were attempted). The sequence output should be divided into files of fastq format, one file for each library with recognizable file names to be related to the input libraries. The sequence data will be put on disk and delivered to USMARC. A minimum of 132 libraries in groups of 36 to 96 will be requested in FY2014, to be sequenced on a minimum of two instrument runs as described above. Remaining library aliquots will be provided to USMARC upon request. This is a buy best value procurement. The criteria are past performance, technical solution, timeliness, and price. The first three criteria combined are of more importance than price. Total Price for sequencing (per run) as described above: _____________________ Total Price per sample for library preparation in groups as described above: ___________ Listed below is the desired timeline for the project: 1. All DNA for the first run will be to the contractor by January 15, 2014. 2. The data will be returned to USMARC a maximum of twelve weeks after the delivery of the DNA. 3. Depending on USMARC research needs, contractor may receive additional sets of DNA throughout the fiscal year, which runs through September 30, 2014 SAM: Vendor must be registered in the System for Award Management prior to the award of the contract. You may register by going to www.sam.gov. You will need to your Tax ID, Duns Number, Bradstreet number and banking information. PAYMENT: Any award made under this solicitation is subject to 31 CFR Part 208 which requires all payments made by the government to be made by electronic funds transfer. Please note on your proposal if you will accept a government purchase (VISA) card. This is a Buy Best Value acquisition. The Government will award a Purchase Order resulting from this Request for Quotes to the responsible offeror whose quote is conforming to the solicitation and represents the most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: The criteria are past performance, technical solution, timeliness, and price. The first three criteria combined are of more importance than price. The Government will evaluate quotes for award purposes, considering the evaluation factors above, and will award to that offeror whose proposal represents the best value to the Government. All sources wishing to provide a quotation must respond by 4:30 PM, December 23, 2014. Quotations should be addressed to USDA Agricultural Research Service, State Spur 18D Bldg 1, Clay Center, NE 68933. POC Donita Furman, Purchasing Agent, +1 (555) 000-0000. Faxed or emailed responses are acceptable. All responses will be evaluated to determine the equipment's capability to meet the above requirements. The Government shall award a contract resulting from this solicitation to the responsive/responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government per FAR 13.106-2.