B--DNA LIBRARY PREP & METABARCODING SEQUENCING

The U.S. Geological Survey’s Conservation Genetics Lab seeks DNA library preparation, next generation sequencing, and custom reference library development for environmental DNA analysis of invasive and native vertebrate and invertebrate species across the United States under a firm fixed-price, one-year contract. Approximately 200 to 300 samples will be processed during the period of performance, with each sample requiring a minimum of 100,000 reads, accurate taxonomic assignment using a reference library with quality assurance and quality control checks, and delivery of raw read files, exact sequence variant tables, Fastq files, and a comprehensive QA/QC report. All deliverables must meet publication-quality standards and be transmitted securely. The contractor is responsible for maintaining sample integrity from receipt through final acceptance by USGS and must manage and dispose of all chemical and biohazardous waste offsite. Laboratory operations must include dedicated, separate spaces for pre-PCR and post-PCR workflows to prevent contamination, with sterile handling protocols for low-concentration DNA samples. The solicitation, numbered 140G0126Q0198, is issued as a Total Small Business Set-Aside under NAICS code 541380, requiring all offerors to be registered in the System for Award Management and to submit completed representations regarding small business status and compliance with federal regulations. Proposals must include technical capability demonstrations and pricing, and will be evaluated under a lowest-price technically acceptable methodology, where only those meeting all mandatory performance and compliance requirements as defined in the performance-based work statement are eligible for award. Quotes are due by July 2, 2026, at 11:00 a.m. Eastern Daylight Time, with an anticipated award date of July 16, 2026. Contract administration is handled by the USGS OAG Reston Acquisition Branch in Reston, Virginia, and all invoices must be submitted electronically through the Treasury’s Invoice Processing Platform within 90 days after performance completion, with funds subject to deobligation 120 days after the period ends. The contract incorporates standard federal clauses on whistleblower protections, prohibitions on restrictive confidentiality agreements, electronic payments, paid sick leave, sustainable products, and supply chain security, and requires full compliance with all FAR representations and certifications at the time of submission.