This Pre-Solicitation opportunity from Department Of Health And Human Services was posted on June 26, 2026. The submission period has ended. Browse the details below for market research, or find similar active opportunities.
Exosome Purification, Exosomal RNA Purification, and Small RNA-Sequencing Services
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The National Institutes of Health, through the Office of the Director and in collaboration with the National Institute on Alcohol Abuse and Alcoholism and the Eunice Kennedy Shriver National Institute of Child Health and Human Development, is seeking specialized laboratory services to support a clinical research study known as POTS, conducted by Dr. Paule V. Joseph at Building 10 in Bethesda, Maryland. The requirement is for a contractor to process 31 Government-furnished plasma specimens through a fully integrated workflow that includes isolation of intact exosomes, purification of exosomal RNA, and small RNA-sequencing on the Illumina platform. Each sample requires precise handling: 0.5 mL of plasma must be processed to yield intact exosomes, split into aliquots with two vials returned to the client, followed by RNA extraction from a secondary aliquot with quality control performed before sequencing. The sequencing must be conducted under strict conditions to produce approximately 20 million raw reads per sample at a 1x51 bp configuration, with full library preparation and quality control included. All data must undergo basic bioinformatics analysis including trimming, filtering, and mapping, followed by delivery via physical hard drive and digital download. Throughout every step, the contractor must maintain specimen integrity and chain of custody to ensure the viability of irreplaceable clinical data. The work must be completed within a timeframe that preserves the scientific integrity of the study, as the receiving laboratory lacks validated internal capacity to perform this complex, time-sensitive process. The solicitation for these services, numbered 75N98026Q00707, was posted on June 26, 2026, with responses due by July 7, 2026. It is classified as a presolicitation under NAICS code 541714 with no set-aside designation, and is open to all qualified providers. The place of performance is in Bethesda, Maryland, and all deliverables—including physical specimen aliquots and digital data outputs—are to be returned to the NIH client. Neither pricing details nor evaluation criteria are specified in the available information, and no contract clauses, special requirements, or formal inspection procedures are outlined beyond the technical scope and quality standards required for process completion.
General Info
Agency
NAICS
Place of Performance
MD, 20892, USASet-Aside
Timeline
Submission Closed
Organization & Contact Information
Full Description
Requirements:
BACKGROUND
With collaboration with the National Institute on Alcohol Abuse and Alcoholism (NIAAA), The National Institutes of Health (NIH), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), requires laboratory services in support of a clinical research study (POTS) in the laboratory of Dr. Paule V. Joseph at the NIH (Building 10, Bethesda, Maryland). The study requires processing of plasma specimens from 31 enrolled human research participants to isolate intact exosomes, purify exosomal RNA, and perform small RNA-sequencing for downstream analysis. These time-sensitive, irreplaceable specimens must be professionally processed to preserve sample integrity and yield analysis-ready data. The requiring laboratory does not have the in-house validated workflow or capacity to perform integrated exosome isolation, exosomal RNA purification, and Illumina small RNA-sequencing to the required standard within the necessary timeframe. The acquisition is therefore pursued to obtain these specialized services from a qualified provider so that the study's analyses can proceed without loss of specimen integrity or previously collected clinical data.
SCOPE OF WORK/TASKS
The contractor shall furnish all labor, equipment, instrumentation, reagents, and materials necessary to isolate intact exosomes, purify exosomal RNA, and perform small RNA-sequencing on the 31 Government-furnished plasma specimens, maintaining specimen integrity and chain of custody throughout.
The following tasks are required for this requirement:
- Purify intact exosomes from 0.5 mL of plasma for each of 31 samples; split the elution into aliquots A and B, and further split aliquot A into two intact-exosome aliquots (two vials per sample returned to the client).
- Purify exosomal RNA from intact exosomes (aliquot B); elute into two RNA aliquots per sample (one returned to the client and one used for small RNA-sequencing); perform QC of the purified RNA.
- Perform small RNA-sequencing for 31 samples, including library preparation, library QC, and sequencing on the Illumina platform (1x51 bp, approximately 20M average raw reads per sample).
- Perform basic bioinformatics analysis (trimming, filtering, and mapping) and transfer all sequencing data to the client via hard drive and digital download.
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