Genome Wide Functional Genomics CRISPR KO Screening Techniques

This contract involves utilizing Cellecta's Next Generation Sequencing (NGS) services to analyze DNA samples generated from CRISPR-based genetic screens conducted by the Functional Genomics Lab at NCATS. The primary objective is to sequence 15 genomic DNA samples derived from a genome-wide CRISPR knockout screen, with the goal of identifying gene regulators that influence cell survival in colorectal cancer models, particularly comparing wild type and topoisomerase-depleted cells. Cellecta is tasked with amplifying and sequencing these samples to an average depth of approximately 500 reads per guide RNA, ultimately providing a detailed spreadsheet report with sgRNA read counts that will help elucidate mechanisms of resistance. The sequencing process is expected to take between six to eight weeks, encompassing DNA amplification, sequencing, and subsequent bioinformatics analysis. The contract was posted on July 17, 2025, with a response deadline of July 24, 2025, and is managed by the NIH's National Center for Advancing Translational Sciences (NCATS) in Rockville, Maryland, with the work performed in Mountain View, California. The procurement is a combined type, with no set-aside restrictions, and is conducted under NAICS code 541380. The designated point of contact for this contract is Brian Lind, reachable via email or phone.