PCR Amplification with Miya et al. (2015) Primers

The contract involves PCR amplification using the Miya et al. (2015) universal vertebrate primers to target mitochondrial DNA for metabarcoding purposes, enabling species identification from environmental samples through high-throughput sequencing. This work is critical for biodiversity monitoring and ecological assessments, leveraging established molecular markers to amplify conserved regions of the mitochondrial genome across a broad range of vertebrate taxa. The subcontract is classified under NAICS code 541380, indicating it falls under scientific research and development services, and is managed by the Office of Acquisition Grants under the Department of the Interior. Proposals must be submitted by August 5, 2026, at 9:00 PM Eastern Time, following a posting date of August 4, 2026. The place of performance and point of contact details are not specified, and no set-aside provisions are indicated, meaning the opportunity is open to all eligible respondents without restriction based on business size or category.