Quantitative Real Time PCR Machine

The U.S. Department of Agriculture, Agricultural Research Service, Pest Management and Biocontrol and Plant Physiology Research units in Maricopa, AZ has a requirement for a has a requirement for a quantitative real time PCR machine. The Pest Management and Biocontrol Research Unit mission is to develop unique and improved biological, behavioral, cultural, and genetic based methods to reduce losses by insects and mites affecting crops in arid land production areas of the U.S. The goals are economically, socially and environmentally acceptable pest population suppression technologies based on the use of behavioral chemicals, genetics, cultural practices, biological control, sampling and decision aids, modeling and population dynamics of pests and their natural enemies. Emphasis is placed on development of fully integrated, ecologically-based management strategies in a changing agricultural environment that includes recognition of the agriculture-urban interface. The mission of the Plant Physiology and Genetic Research Unit is to increase the productivity, profitability and sustainability of agriculture in arid environments by developing new germplasm for both traditional and alternative crops and by improving plant responses to abiotic stresses and to changes in the global environment. Duns Number: Contractor must be registered in CCR Shipping Cost: FOB Destination Preferred Method; however if you choose to quote FOB Origin, please provide estimated shipping cost. Delivery Schedule: Please provide your estimated delivery date with your quotation Evaluation: Quotations received in response to this synopsis/solicitation shall be evaluated on best value to the government. Continent upon availability of funds Brand Name or Equal- Applied Biosystems ViiA 7 Real-Time PCR System Specifications High-throughput real-time PCR capable in 384-well format. Multiplex capable with six decoupled excitation and emission filter channels allowing for maximum number of dye combinations and multiplexing capabilities. Multi-component algorithm that allows for discrimination of cross-channel fluorescence emission. High resolution and sensitivity - detect as small as 1.5-fold changes in target quantities in singleplex reactions and as few a single copy. Quick block changes - easy access to interchangeable thermal cycling block formats. Instrument touch-screen interface - provides one-touch protocols for fast and easy assay setup. Compatible with future automation upgrades including TaqMan Array Cards, a micro fluidic device that incorporates 384 reaction chambers arrayed in a standard 384-well format. Data recovery capability for 100+ runs. Functional and user friendly software - includes quick start feature, email notification, and advanced data analysis modules, with automatic standard curve generation and quantification of unknowns (absolute quantification), melt curve analysis, allelic discrimination (precision melt software), end point fluorescence analysis and gene expression analysis. High Resolution Melt (HRM) Analysis Software. Compatible with predefined TaqMan Array Micro Fluidic Cards. Technical support - includes installation and training and online trouble-shooting/technical support. 3 year extended warranty. ViiATM 7 Real-Time PCR System Specifications Block Change Design Block change from front of instrument in less than 1 min; no secondary tools required Temperature Uniformity - 0.5'C, within 30 sec of reaching temperature set point Run Time ~35 min for 96/384-well blocks, using Applied Biosystems Fast Master Mix Demonstrated Sensitivity Down to 1 copy Dynamic Range Up to 9 logs of linear dynamic range Supported Applications Standard curve, Comparative Ct, Genotyping, Presence/Absence, HRM, Melt curve Resolution Detect as little as 1.5-fold changes in target quantities in singleplex reaction Supported Blocks and Volume 96-well standard (10-100 ?L reactions) 96-well Fast (10-30 ?L reactions) 384-well (5-20 ?L reactions) TaqMan Array Micro Fluidic Cards (~1 ?L reactions) Excitation/Detection Wavelengths 6 excitation (450-670 nm) 6 emission (500-720 nm) 21 CFR p11 Enablement Security, auditing, and eSigniture features for instrument data Power 100-240 V, ~50/60 Hz Automation Includes plate-loading arm w/ barcode (Sales, service, and support provided by Life Technologies) Remote Monitoring Ability to monitor 15 instruments at a time; optional Smart Monitoring Service for remote instrument diagnostic monitoring by Life Technologies Remote Notification Emails user when run is started, stopped, or aborted Data Export Format Fully configurable by user Dimension (W x D x H) 53.3 cm (21 in) x 63.5 cm (25 in) x 64.5 cm (25.4 in) Weight 60.7 kg (134 lb) All quotes are due no later than Wednesday, August 3, 2011 by 12:00 pm Mountain Standard Time by email sheri.marcelo@ars.usda.gov or fax (520) 316-6329. Attachment SF-18 The following Federal Acquisition Clauses apply to this solicitation: 52.204-7 Central Contractor's Registration (April 2008 52.212-4 Contract Terms and Conditions-Commercial Items (June 2010) 52.212-5Contract Terms & Conditions Required to Implement Statues or Executive Orders-Commercial Items (May 2011) 52.223-15 Energy Efficiency in Energy-Consuming Products (Dec 2007) Offerors may obtain full text versions of these clauses electronically at https://www.acquisition.gov/far/