RNA sequencing of adipose and muscle tissue

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation and a separate written solicitation will not be issued. This solicitation number is NIHLM2015523 and is issued as a Request for Quotation (RFQ). The solicitation/contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular 2005-72. The North American Industry Classification (NAICS) Code is 541711 with a size standard of Less than 500 employees. This acquisition is being conducted using Simplified Acquisition Procedures in accordance with FAR Part 13. The National Institutes of Health (NIH), National Library of Medicine (NLM), NIDDK Acquisitions Branch has a requirement to procure testing services to perform RNA-Seq 48 samples (24 Muscle and 24 Adipose). The National Institutes of Health (NIH), National Library of Medicine (NLM), NIDDK Acquisitions Branch, The NIDDK Obesity and Diabetes Clinical Research Center is currently doing a study investigating the etiology of obesity and diabetes. The recent work from protocol 08DKN140, "Predicting Adaptive Thermogenesis" has uncovered a thrifty phenotype of less energy efficient, obese individuals who lose less weight during 6 weeks of supervised caloric restriction. The future success of dietary weight loss efforts may be influenced by the energy expenditure response to caloric restriction. In order to further investigate the etiology of this phenotype, the lab is interested in determining if there are differences in baseline adipose and muscle tissue efficiencies and/or changes in adipose tissue efficiencies in the 12 volunteers from our pivotal study. In this regard, RNA sequencing (RNA-seq) of adipose and muscle tissue obtained through biopsy before and after weight loss. RNA seq, or whole transcriptome shotgun sequencing, uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time. The lab does not have the capacity to perform RNA-Seq at the ODCRS and is looking for a laboratory that can analyse 1 ug of RNA from 48 samples total from our 12 volunteers (24 Muscle and 24 Adipose Tissues). Analysis will be from 1 ug of RNA from 48 samples total from our 12 volunteers (24 Muscle and 24 Adipose Tissues). The lab will use a 2x100 bp Hi-Seq module for 6 sampler per lane with ta total RNA of 1 ug of each sample. Data will be provided to the lab in the form of an Excel spreadsheet within 60 days. It important that this lab has experience in working in informatics who can help us to interpret the data. This service is to be set-up as a single acquisition. The offeror must include a completed copy of the following provisions: 1) FAR Clause 52.212-1 Instructions to Offerors - Commercial items; 2) FAR Clause 52.212-2, Evaluation - Commercial Items. As stated in FAR Clause 52.212-2 (a) The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be advantageous to the Government, price and other factors considered. The following factors will be used to evaluate offers: Technical capability of the services offered to meet the needs of the Governments requirement, Price and Past Performance. Technical Capability will have the greater weight in evaluation. Note: Past Performance Information: Vendors must submit a listing of the most recent contracts/awards (minimum of 3) which demonstrate similar work in nature to this Solicitation. Contracts/awards may include those entered with the Federal Government, state and local governments and commercial concerns. Include the following information for each contract or subcontract: 1. Name of Contracting Organization 2. Contract Number (for subcontracts provide the prime contract number and the subcontract number) 3. Contract Type 4. Total Contract Value 5. Description of Requirement 6. Contracting Officer's Name and Telephone Number 7. Program Manager's Name and Telephone Number 3) FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items; 4) FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; 5) FAR Clause 52-212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions. The Dun and Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN) and the certification of business size shall be included. The clauses are available in full text at http://www.arnet.gov/far. PLEASE NOTE: In order to receive an award, contractor must be registered and have valid certification in the System for Award Management (SAM) http://www.sam.gov. Interested vendors capable of providing the Government with the items specified in this synopsis should submit their quotation to the below address. Quotations will be due on August 31, 2015 at 11:00 PM EST. The quotation must reference Solicitation number NIHLM2015523. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Quotations must be submitted in writing to the National Institutes of Health, National Library of Medicine, 6707 Democracy Blvd., Suite 700W, Bethesda, MD 20892, Attn: Linda Smith. (contact@example.gov) Faxed copies will not be accepted. Email submission is allowed.