RNA Sequencing Services

Document Type: Combined Synopsis/Solicitation Solicitation Number: RFQ-NIAID-2086480 Posted Date: August 4, 2021 Response Date: August 11, 2021 Set Aside: None NAICS Code: 541380 Classification Code: Q301 Contracting Office Address: Department of Health and Human Services, National Institutes of Health, National Institute of Allergy & Infectious Diseases/AMOB, 903 S 4th Street, Hamilton, MT 59840 Primary Point of Contact: Dana Monroe; contact@example.gov Description: This notice is a combined synopsis/solicitation for commercial items using Simplified Acquisition Procedures. This acquisition will be made in accordance with the format in Federal Acquisition Regulation (FAR) Subpart 12.6 - Streamlined Procedures for Evaluation and solicitation for commercial Items, as applicable, and as supplemented with additional information included in this notice. This announcement constitutes the only solicitation (a written solicitation will not be issued) and quotes are being requested. The Solicitation number is RFQ-NIAID-2086480 and the solicitation is issued as a Request for Quotes (RFQ). This acquisition will be processed under FAR Part 13 Simplified Acquisition Procedures (SAP). The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2021-06 July 12, 2021. The North American Industry Classification System (NAICS) code for this procurement is 541380, Testing Laboratories, with a small business size standard of $16.5 million. The requirement is being competed full and open competition, un-restricted and without a small business set-aside. The National Institute of Allergy and Infectious Diseases (NIAID) has a requirement for: Line 1: Library QC, Qty 100 each Line 2: NovaSeq PE150 (1 Lane), Qty 10 each Line 3: Data quality control, Qty 10 each Line 4: Index demultiplexing, Qty 10 each Line 5: FTP, Qty 1 each This requirement is to send 100 samples of RNA from single cells from cell culture and mouse tissue that are Ehrlichia-infected and uninfected controls single cell RNA-sequencing to evaluate the transcriptional profile of arthropod cells infected with Ehrlichia and the cutaneous immune response during tick transmission of Ehrlichia. We require the NovaSeq PE150 read size or larger (larger fragments of DNA map to the genome with fewer false positive errors than smaller fragments) We need a lab that is operating at full capacity now- during the COVID19 crisis-most companies that run labs in the USA are not fully staffed now. We need a company that is willing to accept samples that were infected with a BSL2 pathogen but have been decontaminated. We need enough depth and breathe of reads for us to - see all the genetic changes. Most companies are selling 6G of raw data, but we need 6G of raw data per sample that will give us the coverage we need. We require a company that can sequence all large groups of samples at the same time- running samples in batches increases - noise in the sequencing that may result in false readings of gene changes We require quantification analysis of the data, which requires bioinformatics knowledge and computers with sufficient processing power to run these analyses We need a company the is reliable, has low error rate and is known by other PIs at NIAID working with infectious diseases. Place of Performance: NIH, 904 S. 4th Street, Hamilton, MT 59840, United States. FOB: Destination The government intends to award a firm fixed price purchase order to the responsible contractor as a result of this RFQ that will include the terms and conditions set forth herein. The award will be based on the following; technical capability to meet the requirements and price. The following FAR provisions apply to this acquisition: FAR 52.212-1 Instructions to Offerors Commercial Items (July 2021) FAR 52.212-3 Offerors Representations and Certifications - Commercial Items (FEB 2021) FAR 52.204-7 System for Award Management (Oct 2018) (Offerors must complete annual representations and certifications on-line at http://www.sam.gov/ in accordance with FAR 52.212-3 Offerors Representations and Certifications- Commercial Items (Please ensure 52.209-2 -- Prohibition on Contracting with Inverted Domestic Corporations--Representation. (Nov 2015) is updated in your SAM record) FAR 52.203-18 Prohibition on Contracting with Entities that Require Certain Internal Confidentiality Agreements - Representation (Jan 2017) 52.204'26 Covered Telecommunications Equipment or Services-Representation (OCT 2020) *** Must complete representation if attached to this solicitation- see attachment 52.204-26 The following FAR contract clauses apply to this acquisition: FAR 52-212-4 Contract Terms and Conditions Commercial Items (OCT 2018) FAR 52-212.5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders Commercial Items (July 2021) *The applicable subparagraphs of FAR52.212-5 are included in the attachment to this posting. FAR 52.232-40 Providing Accelerated Payments to Small Business Subcontractors (Dec 2013) FAR 52.204-13 System for Award Management Maintenance (Oct 2018) FAR 52.204-25 Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment (Aug 2020) HHSAR 352.222-70, Contractor Cooperation in Equal Employment Opportunity Investigations (December 2015) 52.252-2 -- Clauses Incorporated by Reference. (Feb 1998) This contract incorporates one or more clauses by reference, with the same force and effect as if they were given in full text. Upon request, the Contracting Officer will make their full text available. Also, the full text of a clause may be accessed electronically at this address: http://farsite.hill.af.mil/vffara.htm By submission of an offer, the offeror acknowledges the requirement that a prospective awardee shall be registered in the System for Award Management (SAM) database prior to award, during performance, and through final payment of any contract, basic agreement, basic ordering agreement, or blanket purchasing agreement resulting from this solicitation (www.sam.gov). Copies of the above-referenced provisions and clauses are available from http://www.acquisition.gov/far/loadmainre.html & http: //www.hhs.gov/regulation/index.html or, upon request, either by telephone or fax. Submission shall be received not later than August 11, 2021 at 5:00pm EST Offers may be e-mailed to Dana Monroe (email: contact@example.gov). Offers shall include RFQ number in the subject line (RFQ-NIAID-2086480). Late submissions shall be treated in accordance with the solicitation provision at FAR 52.212-1(f) All responsible sources may submit an offer that will be considered by this Agency. Any questions or concerns regarding this solicitation should be forwarded in writing via e-mail to Dana Monroe at contact@example.gov.