Shotgun Metagenomic Library Preparation

The contract requires the preparation of double-stranded DNA libraries from 200 biological samples using the skim-Seq methodology, specifically for downstream shotgun metagenomic sequencing. This work is part of a subcontract under the National Institutes of Health, operating through the Department of Health and Human Services, with performance targeted at Bethesda, Maryland, 20892. The project demands high precision in library preparation to ensure accurate representation of microbial communities across all samples, adhering to standardized protocols suitable for large-scale metagenomic analysis. All deliverables must meet stringent quality controls to facilitate seamless sequencing and data interpretation. The solicitation was posted on May 13, 2026, with a response deadline of May 23, 2026, at 4:00 PM Eastern Time. The North American Industry Classification System code 541990 categorizes this as other scientific and technical consulting services, indicating the work falls under specialized biotechnical service provision rather than traditional laboratory manufacturing. There is no set-aside designation, and the posting does not specify a point of contact, suggesting applicants should direct inquiries through the SAM.gov platform linked in the document. Successful subcontractors will need to demonstrate experience with skim-Seq workflows and the ability to handle high-volume sample processing under consistent, reproducible conditions.