DNA Isolation Equipment

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be used. Solicitation number AG737375 is issued as a request for quote (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-80 correction. North American Industrial Classification Code (NAICS) 423490 is applicable. SIC code is 5049. The Small Business size standard is 100 employees for the NAICS code. This procurement small business set aside is dissolved. The United States Department of Agriculture, Agricultural Research Service, Plant Science Research Unit, Raleigh, North Carolina requires an instrument for isolation of high quality genomic DNA. This equipment would be used to isolate DNA for large numbers of samples in conjunction with the Plant Science Research Unit's genetic investigation of complex traits of plants. In order to determine the genetic basis of quantitative traits, DNA is isolated and marker analyses performed for thousands of 96-well plates each year. No. Description of Services Quantity COST 0001 KBS-0009-001 oKtopure High 1 EA $_______ Throughput DNA extraction robot OR EQUAL 0002 KBS-0009-003 oKtopure off line 1 EA $_______ Tip Wash option OR EQUAL Note that all "or equal" submissions shall submit documentation to support the "or equal" claim. Minimum Equipment Salient Features are as follows: - 1 1. Performs walk away purification of genomic DNA from lysed plant tissue. - 2 2. Compatible with sBeadex plant DNA extraction kits (for example - LGC genomics NAP41601 and NAP41640) for nucleic acid purification without ethanol washing to reduce organic waste. 3. Process up to eight 96 well plates in parallel and perform up to 5,000 extractions in 8 hours. The nucleic acids must be eluted into a 96-well plate. 4. Operates with various 96-well formats including deep well blocks, standard microtitre plates, and PCR plates. 5. Tip washing capacity so that tips may be reused multiple time resulting in reduced consumable waste and costs. Wash station and decontamination must eliminate carryover of DNA. 6. Total run time for genomic DNA isolation should be 2 hour or less for 786 samples, with 15 minutes or less of hands-on preparation of samples and reagents 7. Eluted DNA must perform well in downstream applications including SSR and KASP genotyping and genotyping by sequencing. 8. DNA concentrations must be uniform and greater than 50ng/ul from 25mg of plant tissue. Preferences: 1. Standard 1-year commercial warranty that includes all parts, labor and travel for one year at no additional cost. 2. Fits on a bench top. The Government anticipates award of a firm fixed price contract. The Government anticipates awarding one contract. The estimated quantities and schedule are provided to give Offerors a better understanding of the size of the job and to evaluate prices. This in no way obligates the Government to purchase these stated quantities. INSPECTON AND ACCEPTANCE TERMS: Items will be inspected and accepted at destination. The Federal Acquisition Regulation (FAR) and the Department of Agriculture Acquisition Regulation (AGAR) provisions and clauses that apply to this acquisition are attached. This acquisition will be conducted using a Lowest Price Technically Acceptable; FAR part 13. therefore, the proposal that is determined to be the lowest priced technically acceptable based on the three evaluation factors will be awarded. Pursuant to FAR 52.212-2, the criteria for evaluation are below. This procurement will be awarded to the vendor on a Lowest Price Technically Acceptable (LPTA) basis: (1) Technical Capability, (2) Cost/Price, and (3) Past Performance. (1) Technical Capability - Will be evaluated on the following criteria and will be no longer than 10 pages using times new roman with a font size of 12. a. Management Capability b. Personnel qualifications c. Salient features/brochure/specifications (2) Cost/Price - Provide breakdown a. Unit cost b. Shipping cost (3) Past Performance - Provide prior 3 years of experience on same or similar projects. Provide no more than 5 recent purchasers of this equipment. Name of equipment. b. Point of contact name, phone number, email address c. Project cost Technical evaluation will be on a go/no-go basis. Past performance evaluation will be Pass/Fail or Neutral if no past performance is provided. Delivery destination is: USDA-ARS Small Grains Genotyping Lab ATTN: 4114 William Hall Dept. of Crop ScienceO Raleigh, NC 27606 All invoices shall be submitted electronically. Questions: any questions about this solicitation must be sent via email to Mr. Brian Brown at brian.brown@ars.usda.gov no later than 4:00 PM Eastern time March 23, 2015. Answers will be posted as an amendment if needed on FBO. Proposals must be received no later than 4:00 PM Eastern Time on March 30, 2015. The successful company must mandatorily be registered in the (System for Award Management) at the web site: www.sam.gov (SAM) and a contractor can call the SAM help desks at 1-866-606-8220.