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Sea Lice Genotyping

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Solicitation1232SA26Q1506Federal

Contract Overview

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The USDA Agricultural Research Service, specifically the National Cold Water Marine Aquaculture Center, is seeking a contractor for DNA extraction and single nucleotide polymorphism (SNP) genotyping of sea lice (Lepeophtheirus salmonis). This effort involves processing approximately 1,344 samples, submitted in increments of 96, using AXIOM LSAL200K 96-ARRAY genotyping on a GeneTitan Instrument or equivalent. The contractor must utilize a fully integrated Laboratory Information Management System (LIMS) for sample tracking and adhere to a minimum sample pass rate of 95%. Deliverables include raw genotyping data in .cel and PLINK formats delivered via a secure electronic portal within 60 days of sample receipt, as well as the return of all unused tissue and DNA samples to the government. This is a Total Small Business Set-Aside under NAICS 541380, with the award structured as a firm-fixed price contract. The period of performance extends from the date of award through December 31, 2026, with a total requirement of 960 plates. The government will utilize a lowest price technically acceptable (LPTA) evaluation method, assessing offerors based on price, technical acceptability, and past performance. Quotes must be submitted to the contracting officer by September 24, 2026, and prices must remain firm for 90 calendar days.

General Info

USDA seeks small business contractor for sea lice DNA extraction and SNP genotyping.

Agency

Department Of Agriculture → USDA Forest ServiceView Agency

NAICS

541380 - Testing Laboratories and ServicesView NAICS

Place of Performance

Bangor, ME, 04401, USA

Set-Aside

SBA

Documents

(4)

Schedule of Items - DNA Extraction and Genotyping

PDFspecifications

Sol_1232SA26Q1506_Amd_0001.pdf

PDF

1232SA26Q1506 - Sea Lice Genotyping

PDFrfq

corrected+-+Schedule+of+Items.pdf

PDF

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Timeline

1 update
PhaseCombined Synopsis
Posted

Combined Synopsis

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Organization & Contact Information

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AgencyDepartment Of Agriculture → USDA Forest Service
Contacts1 person available
OfficeWASHINGTON, DC, 20250, USA
Organization / Agency
Department Of Agriculture → USDA Forest Service
View Agency Profile
Office AddressWASHINGTON, DC, 20250, USA
Contacts

Full Description

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The USDA Agricultural Research Service requires DNA extraction from sea lice (Lepeophtheirus salmonis) and single nucleotide polymorphism (SNP) genotyping.  Lice tissue samples will consist of single pre-adult or adult animals that have been preserved in ethanol.   Sea lice DNA genotyping will be completed using AXIOM LSAL200K 96-ARRAY genotyping in the 96-well format (Thermo-Fisher SKU number 550522) on the GeneTitan Instrument or equal. Deliverables will include the genotyping data for all the samples and the raw data files in .cel format (CEL files) and PLINK format (either .ped and .map or .bed, .bim, and .fam).  Unused tissue samples and DNA samples will be sent back to the government.  Contractor shall provide all services, supplies, equipment and facilities necessary to provide the deliverable specified in accordance with the terms, conditions, and specifications contained in this document.         

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