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Notice of Intent to Sole Source - 10X Genomics

Awarded

NOI-CC-25-008681Federal

Contract Overview

Solicitation details, issuing organization, response deadlines, documents, and interested companies for this government contract opportunity.

AI Contract Overview

The contract pertains to a notice of intent for a sole source procurement of 10X Genomics single cell sequencing kits, including 5’ v3 kits, TCR amplification kits, and Single Cell Multiome ATAC + Gene Expression kits. These kits contain proprietary reagents necessary for isolating and amplifying uniquely barcoded RNA and chromatin accessible regions from individual cells, enabling detailed profiling of immune responses at the single-cell level. The 10X Genomics platform utilizes microfluidics technology to encapsulate individual cells with gel beads, allowing the labeling and sequencing of RNA from distinct cells. The kits are essential for ongoing research studying the immune response to Pneumocystis infection in mouse models, focusing on gene expression changes and histone modifications in lung tissue samples over time. The 10X Genomics reagents and platform are exclusively manufactured by 10X Genomics and are incompatible with reagents from other manufacturers, making them indispensable for the current study. The Critical Care Medicine Department has already modified and validated the 10X Genomics protocols for their research, and any deviation in equipment or reagents would necessitate a lengthy re-validation process of up to one year. Therefore, no alternative single cell RNA sequencing products can be used without jeopardizing the integrity and timeline of the ongoing study. The contract is issued by the National Institutes of Health, Department of Health and Human Services, with performance based in Bethesda, Maryland.

General Info

Sole source purchase of 10X Genomics single-cell sequencing kits for Pneumocystis immune response research.

Agency

Department Of Health And Human Services → National Institutes Of Health - Cc

NAICS

325413 - In-Vitro Diagnostic Substance Manufacturing

Place of Performance

Bethesda, MD, 20892, USA

Set-Aside

NONE

Documents

Sole Source Justification for 10X Genomics Single Cell Sequencing Kits

justification-and-authorization

Low

AI Contract Breakdown

Uniform Contract Format

Timeline

2 updates

PhaseAwarded

PostedAug 27

special-notice

Notice updatedAug 27

Notice details unchanged. Attachments may have changed.

Notice updatedSep 2

Notice details unchanged. Attachments may have changed.

AwardedOct 29

Contract was awarded

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Organization & Contact Information

AgencyDepartment Of Health And Human Services → National Institutes Of Health - Cc

Contacts1 person available

OfficeBETHESDA, MD, 20892, USA

Organization / AgencyDepartment Of Health And Human Services → National Institutes Of Health - Cc

Office AddressBETHESDA, MD, 20892, USA

Contacts

Chris Ryssa Nix

contact@example.gov

Full Description

This is a notice of intent, not a request for a quotation. A solicitation document will not be issued and quotations will not be requested. This procurement is for 10X Genomics 5 v3 single cell sequencing kits, TCR amplification kits, and Single Cell Multiome ATAC + Gene Expression kits. The kits are composed of reagents for the isolation and amplification of uniquely barcoded ribonucleic acid (RNA) and chromatin accessible regions from individual cells. The 10X Genomics single cell sequencing of immune cells enables high-throughput simultaneous and accurate determination of the nature of an immune response to an infection (what the cells are doing and which cells are doing it). The 10X Genomics platform uses microfluidics to separate individual cells into an emulsion with single gel beads and labels the RNA from each cell with a unique barcode for partitioning into libraries for sequencing. The single cell sequencing kit is proprietary to 10X Genomics, the original equipment manufacturer of the 10X Genomics platform. These supplies will be used for a study of the immune response to Pneumocystis infection in mouse animal models, as well as studying histone modifications, to better under the Pneumocystis biology. Animals will be infected with the fungal pathogen using a co-housing exposure model, and then followed up overtime, collecting lung tissue samples which will be used to assess changes in gene expression overtime. Single cell sequencing will allow for the profiling of immune cell subsets and B cell receptor sequences during infection, and gene expression changes in the fungal pathogen. 10X Genomics is the sole manufacturer of the 10X Genomics platform and single cell sequencing reagents. Other manufacturer's single cell single reagents are not compatible for use with the 10x Genomics platform. The Critical Care Medicine Department has independently modified and validated the recommended protocols provided by the 10X Genomics. Any change in equipment or reagents currently approved for this study and in use under the existing protocols, would negatively impact our current research work. It would require the entire protocol process and program study to undergo the re-validation process which can take up to one year, and so alternative single cell RNA sequencing products from other manufacturers cannot be substituted.