PacBio CCSExpress Library Preparation and Revio Sequencing

This contract requires the preparation of PacBio CCSExpress libraries and the performance of long-read sequencing using the PacBio Revio platform or an equivalent system, with all work to be performed at Kearneysville, West Virginia, ZIP code 25430. The effort is classified as a subcontract under a Small Business Set Aside - Total, meaning only small businesses are eligible to compete, and the North American Industry Classification System code is 541690, which pertains to other scientific and technical consulting services. The opportunity was posted on July 31, 2026, with a response deadline of August 14, 2026, at 2:00 PM Eastern Time. The awarding agency is the USDA Agricultural Research Service, specifically the Appalachian Fruit Research Station, though no point of contact is provided in the documentation. All deliverables must meet the technical specifications for CCSExpress library preparation, which involves generating highly accurate long-read sequencing data through circular consensus sequencing, and must be completed using either the PacBio Revio system or another platform deemed functionally equivalent by the contracting entity. The work is not subject to a specific delivery schedule beyond the proposal deadline, but the performance location is fixed and no alternative sites are permitted. Interested small businesses must submit their responses by the stated deadline to be considered, and failure to comply with the set-aside requirements or provide evidence of small business status will result in disqualification. The contract is part of a broader USDA research initiative aimed at advancing genomic technologies, and the resulting data will support agricultural and biological research activities under the auspices of the agency.